Jeffrey L. Brodsky

  • Avinoff Professor of Biological Sciences
  • Protein quality control

Contact

Office: (412) 624-4831
Lab: (412) 624-4830
A320 Langley Hall
4249 Fifth Avenue
Pittsburgh, PA 15260

Our work focuses on understanding how misfolded proteins are recognized and destroyed in the cell, how molecular chaperones mediate protein quality control “decisions”, how cellular stress impacts protein homeostasis ("proteostasis"), and how defects in protein architecture can be corrected. Our early work contributed to the discovery of the endoplasmic reticulum associated degradation (ERAD) pathway, which we named, and ongoing studies are deciphering the mechanisms underlying this pathway in yeast, mammalian cell culture, and rodent models. The importance of ERAD is evidenced by the fact that >70 human diseases are associated with the pathway, and numerous ERAD substrates play vital roles in human physiology. To determine the molecular basis of these diseases and other protein conformational diseases, we express specific protein substrates in yeast, tissue culture, and rodent models, and characterize factors mediating their folding and disposal--and concomitant stress responses--using genetic, biochemical, cellular, and pharmacological tools. In parallel, biochemical assays are being developed in which distinct steps during ERAD and protein biogenesis can be reconstituted. New classes of small molecule modulators of chaperones and the ubiquitin-proteasome pathway have also been isolated and show efficacy in a range of disease models.

E-mail Lab

Sannino, S., A.M. Manuel, C. Shang, S.G. Wendell,

Sannino, S., A.M. Manuel, C. Shang, S.G. Wendell, P. Wipf , and J.L. Brodsky. Non-essential amino acid availability influences proteostasis and breast cancer cell survival during proteotoxic stress.. Mol. Cancer Res. (2023), In press

Nguyen, N.H. and J.L. Brodsky. The cellular pathwa

Nguyen, N.H. and J.L. Brodsky. The cellular pathways that maintain the quality control and transport of diverse potassium channels. Biochim. Biophys. Acta (2023) 1866: 194908

Farinha, C.M., J.L. Brodsky, and N. Pedemonte. Fun

Farinha, C.M., J.L. Brodsky, and N. Pedemonte. Fundamental and translational research in cystic fibrosis—why we still need it. J. Cyst. Fibr. (2023) 22: S1-S4

Shao, H., S. Taguwa, L. Gilbert, A. Shkedi, S. San

Shao, H., S. Taguwa, L. Gilbert, A. Shkedi, S. Sannino, C.J. Guerriero, Z.T. Young, J.L. Brodsky, J.S. Weissman, J.E. Gestwicki, and J. Frydman. A campaign targeting a conserved Hsp70 site uncovers how subcellular localization is linked to distinct biological activities. Cell Chem. Biol. (2022) 29: 1303-1316

Brodsky J.L., D.M. Engleman, L.M. Hendershot, S. P

Brodsky J.L., D.M. Engleman, L.M. Hendershot, S. Piana-Agostinetti, and T. Sommer. Taking out the trash: How misfolded proteins are removed from the endoplasmic reticulum. Fac. Rev. (2022) 11: 29

Porter, A.W., J.L. Brodsky, and T.M. Buck. Emergin

Porter, A.W., J.L. Brodsky, and T.M. Buck. Emerging Links Between Endoplasmic Reticulum Stress Responses and Acute Kidney Injury. Am. J. Physiol-Cell Physiol. (2022) 323: C1697-C1703

Nakatsukasa, K., S. Wigge, Y. Takano, T. Kawarasak

Nakatsukasa, K., S. Wigge, Y. Takano, T. Kawarasaki, T. Kamura, and J.L. Brodsky. A positive genetic selection for transmembrane domain mutations in HRD1 underscores the importance of Hrd1 complex integrity during ERAD. Curr. Genet. (2022) 68: 227-242

Porter, A., D.N. Nguyen, D. Clayton, W.G. Ruiz, S.

Porter, A., D.N. Nguyen, D. Clayton, W.G. Ruiz, S.M. Mutchler, E.C. Ray, A.L. Marciszyn, L.J. Nkashama, A.R. Subramanya, S. Gingras, T.R. Kleyman, G. Apodaca, L.M. Hendershot, J.L. Brodsky, and T.M. Buck. The GRP170 molecular chaperone protects against renal tubule damage, ER stress, and acute kidney injury. JCI Insight (2022) 7: e151869

Galvin, J., E. Curran, F. Arteaga, A. Goossens, N.

Galvin, J., E. Curran, F. Arteaga, A. Goossens, N. Aubuchon-Endsley, M.A. McMurray, J. Moore, K.C. Hansen, H. Chial, H. Potter, J.L. Brodsky, and C.M. Coughlan. Proteasome activity modulates amyloid toxicity. FEMS Yeast Res. (2022) 22: 1-13

Kumari, D., E.A. Fisher, and J.L. Brodsky. Hsp4

Kumari, D., E.A. Fisher, and J.L. Brodsky. Hsp40s play distinct roles during the initial stages of apolipoprotein B biogenesis. Mol. Biol. Cell (2022) 33: 1-15

Needham, P.J., C.A. Bertrand, J.L. Goeckeler-Fr

Needham, P.J., C.A. Bertrand, J.L. Goeckeler-Fried, C. Zhang, Z. Sun, A.R. Wetzel, and J.L. Brodsky. SLC6A9 is selected for endoplasmic reticulum associated degradation (ERAD) due to Hsp70-dependent targeting of the STAS domain. Biochem. J. (2021) 478: 4203-4220

Bhatia, T.N., R.N. Clark, P.G. Needham, K.M. Mi

Bhatia, T.N., R.N. Clark, P.G. Needham, K.M. Miner, A.S. Jamenis, E.A. Eckhoff, N. Abraham, X. Hu, P. Wipf, K.C. Luk, J.L. Brodsky, and R.K. Leak. Heat Shock Protein 70 as a Sex-Skewed Regulator of α-Synucleinopathy. Neurotherapetics (2021) 18: 2541-2564

Shi, S., T.M. Buck, A.J. Nickerson, J.L. Brodsk

Shi, S., T.M. Buck, A.J. Nickerson, J.L. Brodsky, and T.R. Kleyman. Paraoxonase 2 is an ER chaperone that regulates expression of the epithelial sodium channel, ENaC. Am. J. Phys. Cell Physiol. (2022) 322: C111-C121

Sun, Z., C.J. Guerriero, and J.L. Brodsky. Substra

Sun, Z., C.J. Guerriero, and J.L. Brodsky. Substrate Ubiquitination Retains Misfolded Membrane Proteins in the Endoplasmic Reticulum for Degradation. Cell Rep. (2021) 36: 109717

Burns, G.D., O. Hillal, Z. Sun, K.R. Reutter, G.M.

Burns, G.D., O. Hillal, Z. Sun, K.R. Reutter, G.M. Preston, A.A. Augustine, J.L. Brodsky, and C.J. Guerriero. The characterization of distinct classes of misfolded proteins uncovers differential effects in yeast compromised for proteasome function. FEBS Lettr. (2021) 595: 2383–2394

Kumari, D. and J.L. Brodsky. The targeting of nati

Kumari, D. and J.L. Brodsky. The targeting of native proteins to the Endoplasmic Reticulum Associated Degradation (ERAD) pathway: An expanding repertoire of regulated substrates. Biomolecules (2021) 11: 1185

Sannino, S., M.E. Yates, M.E. Schurdak, S. Oesterr

Sannino, S., M.E. Yates, M.E. Schurdak, S. Oesterreich, A.V. Lee, P. Wipf, and J.L. Brodsky. Unique integrated stress response sensors regulate cancer cell susceptibility when Hsp70 activity is compromised. eLife (2021) 10: e64977

Ferro-Novick, S., F. Reggiori, and J. L. Brodsky.

Ferro-Novick, S., F. Reggiori, and J. L. Brodsky. ER-phagy, ER homeostasis and ER quality control: implications for disease and beyond. Trends Biochem. Sci. (2021) 46: 630-639.

Rastelli, E.J., S. Sannino, D.J. Hart, E.R. Sharlo

Rastelli, E.J., S. Sannino, D.J. Hart, E.R. Sharlow, J.S. Lazo, J.L. Brodsky, and P. Wipf. Synthesis and Evaluation of Bifunctional PTP4A Phosphatase Inhibitors Activating the ER Stress Pathway. Bioorg. Med. Chem. Lettr. (2021) 46: 128167

Goeckeler-Fried, J.L., R.A. Denny, D. Joshi, C. Hi

Goeckeler-Fried, J.L., R.A. Denny, D. Joshi, C. Hill, M.B. Larsen, A.N. Chiang, R.A. Frizzell, P. Wipf, E.J. Sorscher, and J.L. Brodsky. Improved correction of F508del-CFTR biogenesis with a folding facilitator and an inhibitor of protein ubiquitination. Bioorg. Med. Chem. Lettr. (2021) 48: 128243

Honer, J., K.M. Niemeyer, C. Fercher, A.L. Diez

Honer, J., K.M. Niemeyer, C. Fercher, A.L. Diez Tissera, N. Jaberolansar, Y. Jafrani, C. Zhou, J.J. Caramelo, A.M. Shewan, B.L. Schulz, J.L. Brodsky, and L.F. Zacchi. TorsinA folding and N-linked glycosylation are sensitive to redox homeostasis. Biochim. Biophys. Acta Mol. Cell. Res. (2021) 1868: 119073

Buck, T.M., X. Zeng, P.S. Cantrell, R.T. Cattley,

Buck, T.M., X. Zeng, P.S. Cantrell, R.T. Cattley, Z. Hasanbasri, M.E. Yates, D. Nguyen, N.A. Yates, and J.L. Brodsky. The Capture of a Disabled Proteasome Identifies Erg25 as a Substrate for Endoplasmic Reticulum Associated Degradation. Mol. Cell. Proteomics (2020) 19: 1896-1909

Estabrooks, S.E. and J.L. Brodsky. Recapitulation

Estabrooks, S.E. and J.L. Brodsky. Recapitulation disease-causing CFTR variant ubiquitination in isolated membranes. Analytic. Biochem. (2020) 604: 113829

Singh, A., N. Vashistha, J. Heck, X. Tang, P. Wipf

Singh, A., N. Vashistha, J. Heck, X. Tang, P. Wipf, J.L. Brodsky and R.Y. Hampton. Direct involvement of Hsp70 ATP hydrolysis in Ubr1-mediated quality control. Mol. Biol. Cell (2020) 31: 2669-2686

Ponzoni, L., N. H. Nguyen, I. Bahar, and J.L. Brod

Ponzoni, L., N. H. Nguyen, I. Bahar, and J.L. Brodsky. Complementary computational and experimental evaluation of missense variants in the ROMK potassium channel. PLoS Comp. Biol. (2020) 16(4):e1007749.

Terrab, L., C.J. Rosenker, L. Johnstone, L.K. Ngo,

Terrab, L., C.J. Rosenker, L. Johnstone, L.K. Ngo, L. Zhang, N.F. Ware, B. Miller, A.C. Topacio, S. Sannino, J.L. Brodsky, and P. Wipf. Synthesis and Selective Functionalization of Thiadiazine 1,1-Dioxides with Efficacy in a Model of Huntington’s Disease. ACS Med. Chem. Lett. (2020) 11:984-990.

Estabrooks, S.K. and J.L. Brodsky. Regulation of C

Estabrooks, S.K. and J.L. Brodsky. Regulation of CFTR biogenesis by the proteostatic network and pharmacological modulators. Int. J. Mol. Sci. (2020) 21: 452 doi:10.3390/ijms21020452

Betegon, M. and J.L. Brodsky. Unlocking the door f

Betegon, M. and J.L. Brodsky. Unlocking the door for ERAD. Nature Cell Biol. (2020) 22: 263-265

Sun, Z. and J.L. Brodsky. Protein quality contr

Sun, Z. and J.L. Brodsky. Protein quality control in the secretory pathway. J. Cell Biol. (2019) 218: 3171-3187

Guerriero, C.J., Y.K. Gomez, G.J. Daskivich, A.

Guerriero, C.J., Y.K. Gomez, G.J. Daskivich, A.A. Augustine, K.F. Weiberth, K. Nakatsukasa, M. Grabe, and J.L. Brodsky Harmonizing experimental data with modeling to predict membrane protein insertion in yeast. Biophys. J., (2019) 117: 668-678.

Cui, Y., S. Parashar, M. Zahoor, P.G. Needham, M.

Cui, Y., S. Parashar, M. Zahoor, P.G. Needham, M. Mari, M. Zhu, S. Chen, F. Reggiori, H. Farhan, J.L. Brodsky, and S. Ferro-Novick. A COPII subunit acts with an autophagy receptor to target endoplasmic reticulum for degradation. Science (2019) 365: 53-60

Needham, P.G., C. J. Guerriero, and J. L. Brods

Needham, P.G., C. J. Guerriero, and J. L. Brodsky. Chaperoning Endoplasmic Reticulum Associated Degradation (ERAD) and Protein Conformational Diseases, in “Cold Spring Harbor Perspectives in Biology: Protein Homeostasis” (2019) 11(8). pii: a033928.

Doonan*, L.M., C.J. Guerriero*, G.M. Preston*,

Doonan*, L.M., C.J. Guerriero*, G.M. Preston*, T.M. Buck, N. Khazanov, E.A. Fisher, H. Senderowitz, and J.L. Brodsky. Hsp104 Facilitates the Endoplasmic Reticulum Associated Degradation of Aggregation-Prone Substrates in Yeast. Protein Sci. (2019) 28: 1290-1306

Zaman, K., J. Knight, F. Hussain, R. Cao, S.K.

Zaman, K., J. Knight, F. Hussain, R. Cao, S.K. Estabrooks, G. Altawallbeh, K. Holloway, A. Jafrii, V. Sawczak, Y. Li, P. Getsy, F. Sun, T. Raffay, C. Cotton, J.L. Brodsky, A. Periasamy, S.J. Lewis, and B. Gaston. S-Nitrosylation of CHIP Enhances F508del CFTR Maturation. Am. J. Resp. Cell Mol. Biol. (2019) 61: 765-775

Werner, H.M., S.K. Estabrooks, G.M. Preston, J.

Werner, H.M., S.K. Estabrooks, G.M. Preston, J.L. Brodsky, and W.S. Horne. Exploring the Functional Consequences of Protein Backbone Alteration in Ubiquitin through Native Chemical Ligation. ChemBioChem (2019) 20: 2346-2350.

Mann, J.R., A.M. Gleixner, J.C. Mauna, E. Gomes, M

Mann, J.R., A.M. Gleixner, J.C. Mauna, E. Gomes, M.R. DeChellis-Marks, P.G. Needham, K.E. Copley, B. Hurtle, N.J. Pyles, B. Portz, C.B. Calder, Z.P. Wills, U.B. Pandey, J.K. Kofler, J.L. Brodsky, A. Thathiah, J. Shorter, and C.J. Donnelly. RNA-binding antagonizes neurotoxic phase transitions of TDP-43. Neuron (2019) 102: 321-338.

Chiang, A.N., M. Liang, A. Dominguez-Meijide, C

Chiang, A.N., M. Liang, A. Dominguez-Meijide, C. Masaracchia, C.S. Mazzone, D.W. Newhouse, N.M. Kendsersky, M.E. Yates, A. Manos-Turvey, P.G. Needham, J.L. Goeckeler, T.F. Outeiro, P. Wipf, and J.L. Brodsky. Synthesis and Evaluation of Esterified Hsp70 Agonists in Cellular Models of Protein Aggregation and Folding. Bioorg. Med. Chem. (2019) 27(1):79-91.

Pabon, N.A., Y. Xia, S. Estabrooks, Z. Ye, A.K. He

Pabon, N.A., Y. Xia, S. Estabrooks, Z. Ye, A.K. Herbrand, E. Süß, R.M. Biondi, V.A. Assimon, J.E. Gestwicki, J.L. Brodsky, C.J. Camacho, and Z. Bar-Joseph. Proteome-scale detection of small-molecule protein interactions using data from gene expression perturbations. PLoS Comp. Biol. (2018) 14(12):e1006651

Sheng, S., J. Chen, A. Mukherjee, M.E. Yates, T

Sheng, S., J. Chen, A. Mukherjee, M.E. Yates, T.M. Buck, J.L. Brodsky, M.A. Tolino, R.P. Hughey, and T.R. Kleyman Multifunctional thumb domain of the epithelial Na+ channel. J. Biol. Chem. (2018) 293: 17582-17592

Sannino, S., C.J. Guerriero, A.J. Sabnis, D. Be

Sannino, S., C.J. Guerriero, A.J. Sabnis, D. Beer-Stolz, C.T. Wallace, S.C. Watkins, T.G. Bivona, and J.L. Brodsky. Select compensatory quality control pathways play an essential role in cancer cell survival after Hsp70 inhibition. J. Cell Sci. (2018) 131(17). pii: jcs217760.

Buck, T.M. and J. L. Brodsky. Epithelial Sodium

Buck, T.M. and J. L. Brodsky. Epithelial Sodium Channel Biogenesis and Quality Control in the Early Secretory Pathway. Curr. Opin. Nephrol. Hypertens. (2018) 27:364-372  

Mackie, T.D. and J.L. Brodsky. Investigating Po

Mackie, T.D. and J.L. Brodsky. Investigating Potassium Channels In Budding Yeast: A Genetic Sandbox. Genetics (2018) 209:637-650

Sun, Z. and J.L. Brodsky. The Degradation Pathw

Sun, Z. and J.L. Brodsky. The Degradation Pathway of a Model Misfolded Protein is Determined by Aggregation Propensity. Mol. Biol. Cell (2018) 29:1422-1434

Joshi, S., T. Wang, T.L.S. Araujo, S. Sharma, J

Joshi, S., T. Wang, T.L.S. Araujo, S. Sharma, J.L. Brodsky, and G. Chiosis. Adapting to stress – chaperome networks in cancer. Nature Rev. Cancer (2018) 18:562-575

Shurtleff, M.J., Itzhak, D.N., Schirle-Oakdale,

Shurtleff, M.J., Itzhak, D.N., Schirle-Oakdale, N.T., Hussmann, J.A., Weizebahn, J., Jonikas, M.C., Costa, E.A., Popova, K.D., Jan, C.H., Sinitcyn, P., Vembar, S.S., Hernandez, H., Cox, J., Burlingame, A., Brodsky, J.L., Frost, A., Borner, G.H.H., and Weissman, J.S. The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. eLife (2018) 7. pii: e37018

Hager, N.A., C.J. Krasowski, T.D. Mackie, A.R.

Hager, N.A., C.J. Krasowski, T.D. Mackie, A.R. Kolb, P.G. Needham, A.A. Augustine, A. Dempsey, C. Szent-Gyorgyi, M.P. Bruchez, D.J. Bain, A.V. Kwiatkowski, A.F. O’Donnell and J.L. Brodsky. Select α-arrestins control surface abundance of the mammalian Kir2.1 potassium channel in a yeast model. J. Biol. Chem. (2018) 293:11006-11021

Preston, G.M., C.J. Guerriero, M.B. Metzger, S.

Preston, G.M., C.J. Guerriero, M.B. Metzger, S. Michaelis, and J.L. Brodsky. Substrate Insolubility Dictates Hsp104-Dependent Endoplasmic Reticulum Associated Degradation. Mol. Cell (2018) 70(2):242-253.e6

Doonan, L., E.A. Fisher, and J.L. Brodsky. Can

Doonan, L., E.A. Fisher, and J.L. Brodsky. Can modulators of Apolipoprotein B biogenesis serve as an alternate target for cholesterol-lowering drugs? Biochim. Biophys. Acta (2018) 1863(7):762-771

Mackie, T.D., B.Y. Kim, A.R. Subramanya, D.J. B

Mackie, T.D., B.Y. Kim, A.R. Subramanya, D.J. Bain, A.F. O’Donnell, P.A. Welling, and J.L. Brodsky. The endosomal trafficking factors CORVET and ESCRT suppress plasma membrane residence of the renal outer medullary potassium channel (ROMK). J. Biol. Chem. (2018) 293: 3201-3217

Amengual, J., L. Guo, A. Strong, J. Madrigal-Ma

Amengual, J., L. Guo, A. Strong, J. Madrigal-Matute, H. Zhang, S. Kaushik, J.L. Brodsky, D.J. Rader, A.M. Cuervo, and E.A. Fisher. Autophagy is required for sortilin-mediated degradation of apolipoprotein B100. Circul. Res. (2018) 122:568-582

Kashlan, O.B., C.L. Kinlough, M.M. Myerburg, S.

Kashlan, O.B., C.L. Kinlough, M.M. Myerburg, S. Shi, J. Chen, B.M. Blobner, T.M. Buck, J.L. Brodsky, R.R. Hughey, and T.R. Kleyman. N-linked Glycans are Required on Distinct Subunits for Epithelial Na+ Channel Maturation and Expression. Am. J. Physiol. Renal Physiol. (2018) 314: F483-F492

Sun, Z. and J.L. Brodsky.  Guardians of th

Sun, Z. and J.L. Brodsky.  Guardians of the ERAD Galaxy.  Cell (2017) 171:267-268.

Sannino, S. and J.L. Brodsky.  Targeting p

Sannino, S. and J.L. Brodsky.  Targeting protein quality control pathways in breast cancer.  BMC Biol. (2017) 15(1):109.

Nakasone, M.A., T.A. Lewis, O. Walker, A. Thaku

Nakasone, M.A., T.A. Lewis, O. Walker, A. Thakur, W. Mansour, C.A. Castañeda, J.L. Goeckeler, F. Parlati, T.-F. Chou, O. Hayat, U.K. Nowicka, S. Krueger, S.L. Schreiber, M.H. Glickman, J.L. Brodsky, R.J. Deshaies, and D. Fushman.  Structural basis for the inhibitory effect of ubistatins. Structure (2017) 25(12):1839-1855

Gleixner, A.M., D.F. Hutchinson, S. Sannino, T.

Gleixner, A.M., D.F. Hutchinson, S. Sannino, T.N. Bhatia, L.C Leak, P.T. Flaherty, P. Wipf, J.L. Brodsky, and R.K. Leak.  N-acetyl-L-cysteine protects astrocytes against proteotoxicity without recourse to glutathione.  Mol. Pharm. (2017) 92:564-575.

Shi, S., T.M. Buck, C.L. Kinlough, A.L. Marcisz

Shi, S., T.M. Buck, C.L. Kinlough, A.L. Marciszyn, R.P. Hughey, M. Chalfie, J.L. Brodsky and T.R. Kleyman.  Regulation of the Epithelial Na+ Channel by Paraoxonase-2. J. Biol. Chem. 292:15927-15938.

Zacchi, L.F., J.C. Dittmar, M.J. Mihalevic, A.

Zacchi, L.F., J.C. Dittmar, M.J. Mihalevic, A. Shewan, B.L. Schulz, J.L. Brodsky, and K.A. Bernstein.  A novel high-throughput yeast genetic screen for factors modifying protein levels of the Early-Onset Torsion Dystonia-associated variant torsinADE. Dis. Mod. Mech. (2017) 10: 1129-1140

O'Donnell, B.M., T.D. Mackie, and J.L. Brodsky.

O'Donnell, B.M., T.D. Mackie, and J.L. Brodsky. Linking channelopathies with endoplasmic reticulum associated degradation.  Channels (2017) 11: 499-501

O’Donnell, B.M., T.D. Mackie, A.R. Subramanya,

O’Donnell, B.M., T.D. Mackie, A.R. Subramanya, and J.L. Brodsky. Endoplasmic Reticulum Associated Degradation of the Renal Outer Medullary Potassium Channel, ROMK, Underlies Type II Bartter Syndrome. J. Biol. Chem. (2017) 292:12813-12827

Guerriero, C.J., K. Reutter, A.A. Augustine, G.

Guerriero, C.J., K. Reutter, A.A. Augustine, G.M. Preston, K.F. Weiberth, T.D. Mackie, H.C. Cleveland, N.P. Bethel, K.M. Callenberg, K. Nakatsukasa, M. Grabe, and J.L. Brodsky. Energetic barriers to the retrotranslocation of integral membrane ERAD substrates. Mol. Biol. Cell (2017) 28:2076-2090

Ye, Z., P.G. Needham, S.K. Estabrooks, S.K. Whi

Ye, Z., P.G. Needham, S.K. Estabrooks, S.K. Whitaker, B.L. Garcia, S. Misra, J.L. Brodsky, and C.J. Camacho.  Symmetry breaking during homodimeric assembly kinetically activates an E3 ubiquitin ligase.  Sci. Rep. (2017) 7: 1789

Braganza, A., J. Li, X. Zeng, N. Yates, N.B. De

Braganza, A., J. Li, X. Zeng, N. Yates, N.B. Dey, J. Andrews, J. Clark, X.-H. Wang, C. St. Croix, R. O’Sullivan, L. Garcia-Exposito, J.L. Brodsky, and R.W. Sobol.  UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligase. J. Biol. Chem. (2017) 292: 2470-2484

Buck, T.M., A.S. Jordahl, M.E. Yates, G.M. Pres

Buck, T.M., A.S. Jordahl, M.E. Yates, G.M. Preston, E. Cook, T.R. Kleyman, and J.L. Brodsky.  Interactions between intersubunit transmembrane domains regulate the chaperone-dependent degradation of an oligomeric membrane protein.  Biochem. J. (2017) 474: 357-376

Preston, G.M., and J.L. Brodsky.  The evol

Preston, G.M., and J.L. Brodsky.  The evolving role of ubiquitin modification in endoplasmic reticulum associated degradation.  Biochem. J. (2017) 474: 445-469

Chung, W. J., J.L. Goeckeler-Fried, V. Havasi,

Chung, W. J., J.L. Goeckeler-Fried, V. Havasi, A. Chiang, S.M. Rowe, Z.E. Plyler, J.S. Hong, M. Mazur, G.A. Piazza, A.B. Keeton, E.L. White, L. Rasmussen, A.M. Weissman, R.A. Denny, J.L Brodsky, and E.J. Sorscher.  Increasing the Endoplasmic Reticulum Pool of the F508DEL Allele of the Cystic Fibrosis Transmembrane Conductance Regulator Leads to Greater Folding Correction by Small Molecule Therapeutics. PLoS ONE (2016) 11(10):e0163615

McClure, M.L., S. Barnes, J.L. Brodsky, and E.J

McClure, M.L., S. Barnes, J.L. Brodsky, and E.J. Sorscher.  Trafficking and function of the cystic fibrosis transmembrane conductance regulator: A complex network of post-translational modifications.  Am. J. Physiol. Lung Cell. Mol. Physiol. (2016) 311: L719-L733

Manos-Turvey, A., H.A. Al-Ashtal, C.B. Hartline

Manos-Turvey, A., H.A. Al-Ashtal, C.B. Hartline, M.N. Prichard, P. Wipf, and J.L. Brodsky. Dihydropyrimidinones and -thiones with improved activity against human polyomavirus family members.  Bioorg. Med. Chem. Lettr. (2016) 26: 5087-5091

Sabnis*, A.J., C.J. Guerriero*, V. Olivas, A. S

Sabnis*, A.J., C.J. Guerriero*, V. Olivas, A. Sayana, J.H. Shue, J. Flanagan, S. Asthana, M. Loh, A.W. Paton, J.C. Paton, J. Gestwicki, P. Walter, J.S. Weissman, P. Wipf, J.L. Brodsky, T.G. Bivona.  Combined chemical-genetic approach identifies cytosolic Hsp70 dependence in rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA (2016) 113: 9015-9020

Veit G, Avramescu RG, Chiang AN, Houck SA, Cai

Veit G, Avramescu RG, Chiang AN, Houck SA, Cai Z, Peters KW, Hong JS, Pollard HB, Guggino WB, Balch WE, Skach WR, Cutting GR, Frizzell RA, Sheppard DN, Cyr DM, Sorscher EJ, Brodsky JL, Lukacs GL. From CFTR biology toward combinatorial pharmacotherapy: expanded classification of cystic fibrosis mutations. Mol Biol Cell. (2016) 27:424-33.

Prosser DC, Pannunzio AE, Brodsky JL, Thorner J

Prosser DC, Pannunzio AE, Brodsky JL, Thorner J, Wendland B, O'Donnell AF.  α-Arrestins participate in cargo selection for both clathrin-independent and clathrin-mediated endocytosis.  J Cell Sci. (2015) 128:4220-34

Brodsky, J.L. and R.A. Frizzell.  A Combin

Brodsky, J.L. and R.A. Frizzell.  A Combination Therapy for Cystic Fibrosis.  Cell (2015) 163: 17

Chen, J., E.C. Ray, M.E. Yates, T.M. Buck, J.L.

Chen, J., E.C. Ray, M.E. Yates, T.M. Buck, J.L. Brodsky, C.L. Kinlough, K.L. Winarski, R.P. Hughey, T.R. Kleyman, and S. Sheng.  Functional Roles of Clusters of Hydrophobic and Polar Residues in the Epithelial Na+ Channel Knuckle Domain. J. Biol. Chem. (2015) 290: 25140-25150

Marcoline, F.V., N. Bethel, C.J. Guerriero, J.L

Marcoline, F.V., N. Bethel, C.J. Guerriero, J.L. Brodsky, and M. Grabe. Membrane protein properties revealed through data-rich electrostatics calculations. Structure (2015) 23: 1526-1537

Manos-Turvey, A., J.L. Brodsky, and P. Wipf. Th

Manos-Turvey, A., J.L. Brodsky, and P. Wipf. The Effect of Structure and Mechanism of the Hsp70 Chaperone on the Ability to Identify Chemical Modulators and Therapeutics, In “Heat Shock Protein Inhibitors: Success Stories”, Springer, Top. Med. Chem. (2015) 81-129

Needham, P.G., H.J. Patel, G. Chiosis, P.H. Thi

Needham, P.G., H.J. Patel, G. Chiosis, P.H. Thibodeau, and J.L. Brodsky.  Mutations in the yeast Hsp70, Ssa1, at P417 alter ATP cycling, interdomain coupling, and specific chaperone functions. J. Mol. Biol. (2015) 427: 2948-2965

An, P., J.L. Brodsky, and J.M. Pipas.  The

An, P., J.L. Brodsky, and J.M. Pipas.  The Conserved Core Enzymatic Activities and the Distinct Dynamics of Polyomavirus Large T Antigens. Arch. Biochem. Biophys. (2015) 573:23-31

Buck, T.M., R. Jordan, J. Lyons-Weiler, J.L. Ad

Buck, T.M., R. Jordan, J. Lyons-Weiler, J.L. Adelman, P.G. Needham, T.R. Kleyman, and J.L. Brodsky. The expression of three topologically distinct membrane proteins elicits unique stress response pathways in the yeast Saccharomyces cerevisiae. Physiol. Genomics (2015) 47: 198-214

Posimo, J.M., J.N. Weilnau, A.M. Gleixner, M.T.

Posimo, J.M., J.N. Weilnau, A.M. Gleixner, M.T. Broeren, N. Weiland, J.L. Brodsky, P. Wipf, and R.K. Leak. Heat shock protein defenses in the neo- and allocortex of the telencephalon. Neurobiol. Aging (2015) 36: 1924-1937

Crum, T.S., A.M. Gleixner, J.M. Posimo, D.M. Ma

Crum, T.S., A.M. Gleixner, J.M. Posimo, D.M. Mason, M.T. Broeren, S.D. Heinemann, P. Wipf, J.L. Brodsky, and R.K. Leak.  Heat shock protein responses to aging and proteotoxicity in the olfactory bulb.  J. Neurochem. (2015) 133: 780–794

Buck, T.M and J.L. Brodsky.  Escaping the

Buck, T.M and J.L. Brodsky.  Escaping the endoplasmic reticulum: Why does a molecular chaperone leave home for greener pastures?  EMBO J. (2015) 34: 1-3

Zacchi, L.F., J.J. Caramelo, A.A. McCracken, an

Zacchi, L.F., J.J. Caramelo, A.A. McCracken, and J.L. Brodsky.  Endoplasmic Reticulum Associated Degradation and Protein Quality Control.  Encyclopedia of Cell Biology (R.A. Bradshaw and P. Stahl, Eds.), Elsevier (2016) 1: 596-611

Wipf, P., Eyer, B.R., Yamaguchi, Y., Zhang, F.,

Wipf, P., Eyer, B.R., Yamaguchi, Y., Zhang, F., Neal, M.D., Sodhi, C.P., Good, M., Branca, M., Prindle, T., Jr., Lu, P., Brodsky, J. L., and Hackam, D. J. Synthesis of anti-Inflammatory α- and β-Linked Acetamidopyranosides as Inhibitors of Toll-Like Receptor 4 (TLR4). Tetrahedron Lett. (2015) 56: 3097-3100

Li, H., H.C. Wu, Z. Liu, L.F. Zacchi, J.L. Brod

Li, H., H.C. Wu, Z. Liu, L.F. Zacchi, J.L. Brodsky, and M. Zolkiewski. Intracellular Complexes of the Early-Onset Torsion Dystonia-Associated AAA+ ATPase TorsinA. SpringerPlus (2014) 3:743

Howe, M.K., K. Bodoor, D.A. Carlson, P.F. Hughe

Howe, M.K., K. Bodoor, D.A. Carlson, P.F. Hughes, D.R. Loiselle, A.M. Jaeger, D.B. Darr, J.L. Jordan, L.M. Hunter, E.T. Molzberger, T.A. Gobillot, D.J. Thiele, J.L. Brodsky, N. Spector and T.A.J. Haystead.  Identification of a Novel Allosteric Small Molecule Inhibitor of the Inducible Form of Heat Shock Protein 70.  Chemistry & Biology (2014) 21: 1648–1659

Brodsky, J.L. and P.L. Clark.  Protein Fol

Brodsky, J.L. and P.L. Clark.  Protein Folding in the Cell, From Atom to Organism.  FASEB J. (2014) 28: 5034-5038

Ireland, A.W., T.A. Gobillot, T. Gupta, S.P. Se

Ireland, A.W., T.A. Gobillot, T. Gupta, S.P. Seguin, M. Liang, L. Resnick, K. Lloyd, M.T. Goldberg, A. Manos-Turvey, J.M. Pipas, P. Wipf, and J.L. Brodsky.  Synthesis and Structure Activity Relationships of Small Molecule Inhibitors of the Simian Virus 40 T Antigen Oncoprotein, an Anti-Polyomaviral Target.  Bioorg. Med. Chem. (2014) 22: 6490-6502

Surlow, B.A., B. Cooley, P.G. Needham, J.L. Bro

Surlow, B.A., B. Cooley, P.G. Needham, J.L. Brodsky, and J. Patton-Vogt.  Loss of Ypk1 accelerates Plb1-mediated phosphatidylcholine deacylation in S. cerevisiae.  J. Biol. Chem. (2014) 289(45):31591-604

Alvaro, C.G., A.F. O’Donnell, D.C. Prosser, A.A

Alvaro, C.G., A.F. O’Donnell, D.C. Prosser, A.A. Augustine, A. Goldman, J.L. Brodsky, M.S. Cyert, B. Wendland, and J. Thorner.  Specific a-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein coupled receptor Ste2.  Mol. Cell. Biol. (2014) 34:2660-81.

Adam, C., A. Baeurle, J.L. Brodsky, P. Wipf, D.

Adam, C., A. Baeurle, J.L. Brodsky, P. Wipf, D. Schrama, J.C. Becker, and R. Houben.  The HSP70 Modulator MAL3-101 inhibits Merkel Cell Carcinoma. PLoS ONE (2014) 9: e92041

Zacchi, L.F., H.C. Wu, S.L. Bell, L. Millen, A.

Zacchi, L.F., H.C. Wu, S.L. Bell, L. Millen, A.W. Paton, J.C. Paton, P.J. Thomas, M. Zolkiewski, and J.L. Brodsky. The BiP molecular chaperone plays multiple roles during the biogenesis of TorsinA, a AAA+ ATPase associated with the neurological disease Primary Torsion Dystonia.  J. Biol. Chem. (2014) 289: 12727-12747

Nakatsukasa, K., T. Kamura, and J. L. Brodsky.&

Nakatsukasa, K., T. Kamura, and J. L. Brodsky.  Recent technical developments in the study of ER-associated degradation.  Curr. Opin. Cell Biol. (2014) 29:82–91

Brodsky, J.L., A. Merz, and T. Serio. Organelle

Brodsky, J.L., A. Merz, and T. Serio. Organelle and Proteome Quality Control Mechanisms: How Cells are able to Keep Calm and Carry On.  Mol. Biol. Cell (2014) 25: 733-734

Hecht, K.A., A.F. O’Donnell, and J.L. Brodsky.

Hecht, K.A., A.F. O’Donnell, and J.L. Brodsky. The proteolytic landscape of the yeast vacuole.  Cell. Logistics (2014) 4(1):e28023

Kang, Y., T. Taldone, H.J. Patel, P.D. Patel, A

Kang, Y., T. Taldone, H.J. Patel, P.D. Patel, A. Rodina, A. Gozman, R. Maharaj, C.C. Clement, M.R. Patel, J.L. Brodsky, J.C. Young, G. Chiosis. Heat shock protein 70 inhibitors. 1. 2,5'-thiodipyrimidine and 5-(phenylthio)pyrimidine acrylamides as irreversible binders to an allosteric site on heat shock protein 70.  J. Med. Chem. (2014) 57:1188-1207.

Brodsky, J.L. The threads that tie protein-fold

Brodsky, J.L. The threads that tie protein-folding diseases.  Dis. Model. Mech. (2014) 7: 3-4

Tran, J. R. and J. L. Brodsky.  The Cdc48-

Tran, J. R. and J. L. Brodsky.  The Cdc48-Vms1 complex maintains 26S proteasome architecture.  Biochem. J. (2014) 458: 459-467

Kolb, A. R., P. G. Needham, C. Rothenberg, C. J

Kolb, A. R., P. G. Needham, C. Rothenberg, C. J. Guerriero, P. A. Welling, and J. L. Brodsky.  ESCRT regulates surface expression of the Kir2.1 potassium channel.  Mol. Biol. Cell (2014) 25: 276- 289

Butkinaree, C., L. Guo, B. Ramkhelawon, A. Wans

Butkinaree, C., L. Guo, B. Ramkhelawon, A. Wanschel, J.L. Brodsky, K.J. Moore, and E.A. Fisher. A Regulator of Secretory Vesicle Size, KLHL12, Facilitates the Secretion of Apolipoprotein B100 and Very Low Density Lipoproteins.  Arterioscler. Thromb. Vasc. Biol. (2014) 34: 251-254

Simon, N., M. L. Bochman, S. Seguin, J. L. Brod

Simon, N., M. L. Bochman, S. Seguin, J. L. Brodsky, W. L. Seibel, and A. Schwacha. Ciprofloxacin is an inhibitor of the MCM2-7 replicative helicase.  Biosci. Rep. (2013) 33(5), art:e00072

Brodsky, J.L.  Just a trim, please: Refini

Brodsky, J.L.  Just a trim, please: Refining ER degradation through deubiquitination.  Cell (2013) 154: 479-481

Guerriero, C. J., K. F. Weiberth, and J. L. Bro

Guerriero, C. J., K. F. Weiberth, and J. L. Brodsky.  Hsp70 Targets a Cytoplasmic Quality Control Substrate to the San1p Ubiquitin Ligase.  J. Biol. Chem. (2013) 288: 18506-18520

Buck, T. M., L. Plavchak, A. Roy, B. F. Donnell

Buck, T. M., L. Plavchak, A. Roy, B. F. Donnelly, O. B. Kashlan, T. R. Kleyman, A. R. Subramanya, J. L. Brodsky. The Lhs1/GRP170 chaperones facilitate the endoplasmic reticulum associated degradation of the epithelial sodium channel.  J. Biol. Chem. (2013) 288: 18366-18380

Neal, M.D., J. Hongpeng, B. Eyer, M. Good, C. J

Neal, M.D., J. Hongpeng, B. Eyer, M. Good, C. J. Guerriero, C. P. Sodhi, A. Afrazi, T. Prindle Jr, C. Ma, M. Branca, J. Ozoleck, T. R. Billiar, J. L. Brodsky, P. Wipf, and D. J. Hackam.  Discovery and Validation of a New Class of Small Molecule TLR4 Inhibitors that Attenuate Endotoxemia and Necrotizing Enterocolitis.  PLoS ONE (2013) 8 (6): e65779

Hecht, K.A., V. Wytiaz, T. Ast, M. Schuldiner,

Hecht, K.A., V. Wytiaz, T. Ast, M. Schuldiner, and J.L. Brodsky.  Characterization of an M28 family member residing in the yeast vacuole.  FEMS Yeast Res. (2013) 13: 471-484

Kilpatrick K, Novoa JA, Hancock T, Guerriero CJ

Kilpatrick K, Novoa JA, Hancock T, Guerriero CJ, Wipf P, Brodsky JL, Segatori L. Chemical induction of Hsp70 reduces α-synuclein aggregation in neuroglioma cells. ACS Chem Biol. (2013) 8: 1460-1468

Needham PG, Brodsky JL. How early studies on se

Needham PG, Brodsky JL. How early studies on secreted and membrane protein quality control gave rise to the ER associated degradation (ERAD) pathway: The early history of ERAD. Biochim Biophys Acta. (2013) 1883: 2447-2457

Nakatsukasa K, Brodsky JL, Kamura T. A stalled

Nakatsukasa K, Brodsky JL, Kamura T. A stalled retrotranslocation complex reveals physical linkage between substrate recognition and proteasomal degradation during ER associated degradation. Mol Biol Cell. (2013) 24: 1765-1775

Andreo U, Guo L, Chirieac DV, Tuyama AC, Monten

Andreo U, Guo L, Chirieac DV, Tuyama AC, Montenont E, Brodsky JL, Fisher EA. Insulin-Stimulated Degradation of Apolipoprotein B100: Roles of Class II Phosphatidylinositol-3-Kinase and Autophagy. PLoS One. 2013;8(3):e57590.

Abisambra J, Jinwal UK, Miyata Y, Rogers J, Bla

Abisambra J, Jinwal UK, Miyata Y, Rogers J, Blair L, Li X, Seguin SP, Wang L, Jin Y, Bacon J, Brady S, Cockman M, Guidi C, Zhang J, Koren J, Young ZT, Atkins CA, Zhang B, Lawson LY, Weeber EJ, Brodsky JL, Gestwicki JE, Dickey CA. Allosteric Heat Shock Protein 70 Inhibitors Rapidly Rescue Synaptic Plasticity Deficits by Reducing Aberrant Tau. Biol Psychiatry. (2013) 74: 367-374

Donnelly BF, Needham PG, Snyder AC, Roy A, Khad

Donnelly BF, Needham PG, Snyder AC, Roy A, Khadem S, Brodsky JL, Subramanya AR. Hsp70 and Hsp90 Multichaperone Complexes Sequentially Regulate Thiazide-Sensitive Cotransporter ER-Associated Degradation and Biogenesis. J Biol Chem. (2013) 288: 13124-13135

Miyata Y, Li X, Lee HF, Jinwal UK, Srinivasan S

Miyata Y, Li X, Lee HF, Jinwal UK, Srinivasan SR, Seguin SP, Young ZT, Brodsky JL, Dickey CA, Sun D, Gestwicki JE. Synthesis and Initial Evaluation of YM-08, a Blood-Brain Barrier Permeable Derivative of the Heat Shock Protein 70 (Hsp70) Inhibitor MKT-077, Which Reduces Tau Levels. ACS Chem Neurosci. (2013) 4: 930-939

Brodsky, J.L. Cleaning Up: ER-Associated Degrad

Brodsky, J.L. Cleaning Up: ER-Associated Degradation to the Rescue. Cell (2012) 151: 1163-1167

Gelling, C. L., I. W. Dawes, D. H. Perlmutter,

Gelling, C. L., I. W. Dawes, D. H. Perlmutter, E. A. Fisher, and J. L. Brodsky.  The endosomal protein sorting receptor sortilin has a role in trafficking alpha-1 antitrypsin.  Genetics (2012) 192: 889-903

Fisher, E. A. and J. L. Brodsky.  The unfo

Fisher, E. A. and J. L. Brodsky.  The unfolded protein response: A multifaceted regulator of lipid and lipoprotein metabolism.  Cell Metab. (2012) 16: 407-408

Seguin, S. P., A. W. Ireland, T. Gupta, Y. Miya

Seguin, S. P., A. W. Ireland, T. Gupta, Y. Miyata, P. Wipf, J. M. Pipas, J. E. Gestwicki, and J. L. Brodsky.  A screen for modulators of large T antigen’s ATPase activity uncovers novel inhibitors of simian virus 40 and BK virus replication. Antiviral Res. (2012) 96: 70-81

Tran, J., and J. L. Brodsky.  Assays to me

Tran, J., and J. L. Brodsky.  Assays to measure ER associated degradation (ERAD) in yeast, in “Ubiquitin family modifiers and the proteasome: Reviews and protocols” (Dohmen, J., and Scheffner, M., Eds.).  Methods Mol. Biol. (2012) 832: 505-518

Hutt, D, M., D. M. Roth, M. Chalfant, R. T. You

Hutt, D, M., D. M. Roth, M. Chalfant, R. T. Youker, J. Matteson, J. L. Brodsky, and W. E. Balch.  (2012) FKBP8 peptidyl-prolyl isomerase activity manages a late stage of CFTR folding and stability.  J. Biol. Chem. (2012) 287: 21914-21925

Arora, R., M. Shuda, A. Gustafierro, H. Feng, T

Arora, R., M. Shuda, A. Gustafierro, H. Feng, T. Toptan, Y. Tolstov, D. Normolle, L. L. Vollmer, A. Vogt, A. Dömling, J. L. Brodsky, Y. Chang, and P. S. Moore.  (2012) Viral knockdown reveals surviving as a therapeutic target for Merkel cell carcinoma.  Science Transl. Med. (2012) 4(133):133ra56

Grubb, S., L. Guo, E. A. Fisher, and J. L. Brod

Grubb, S., L. Guo, E. A. Fisher, and J. L. Brodsky. (2012) Protein Disulfide Isomerases Contribute Differentially to the Endoplasmic Reticulum – Associated Degradation of Apolipoprotein B and Other Substrates.  Mol. Biol. Cell (2012) 23(4):520-32

Guerriero, C., and J. L. Brodsky. (2012) On the

Guerriero, C., and J. L. Brodsky. (2012) On the precipice: the delicate balance between secreted protein folding and endoplasmic reticulum-associated degradation (ERAD) in human physiology. Physiol. Rev. (2012) 92: 537-576

Needham, P. G., K. Mikoluk, P. Dhakarwal, S. Kh

Needham, P. G., K. Mikoluk, P. Dhakarwal, S. Khadem, A. C. Snyder, A. R. Subramanya, and J. L. Brodsky. (2011)  The Thiazide-Sensitive NaCl Cotransporter is Targeted for Chaperone-Dependent ER-Associated Degradation. J. Biol. Chem. 286:43611-21

Daghestani, H.N., G. Zhu, P.A. Johnston, S. Shi

Daghestani, H.N., G. Zhu, P.A. Johnston, S. Shinde, J.L. Brodsky, and B.W. Day.  (2011)  Characterization of inhibitors of glucocorticoid receptor nuclear translocation: A model of cytoplasmic dynein-mediated cargo transport. Assay Drug Devel. Technol. 10:46-60

Braunstein, M. J., S. S. Scott, C. M. Scott, S.

Braunstein, M. J., S. S. Scott, C. M. Scott, S. Behrman, P. Walter, P. Wipf, J. D. Coplan, W. J. Chirico, D. Joseph, J. L. Brodsky, and O. Batuman.  (2011)  Cytotoxic effects of the Hsp70 molecular chaperone inhibitor MAL3-101 on multiple myeloma.  J. Oncol. 2011:232037

Seguin, S. P., C. W. Evans, M. Nebane, S. McKel

Seguin, S. P., C. W. Evans, M. Nebane, S. McKellip, S. Ananthan, N. A. Tower, M. Sosa, L. Rasmussen, E. L. White, B. E. Maki, D. S. Matharu, J. E. Golden, J. Aubé, J. L. Brodsky, and J. W. Noah. (2011)  High-throughput Screening Identifies A Bisphenol Inhibitor of SV40 Large T-Antigen ATPase Activity. J. Biomol. Screen. 17:194-203

Falcone, D., M. P. Henderson, H. Nieuwland, C. M. Coughlan, J. L. Brodsky, and D. W. Andrews.&nbs

Falcone, D., M. P. Henderson, H. Nieuwland, C. M. Coughlan, J. L. Brodsky, and D. W. Andrews.  (2011)  Stability and function of the Sec61 translocation complex depends on the Sss1p tail-anchor sequence.  Biochem. J. 436: 291-303

Brodsky, J. L., and W. R. Skach. (2011) Protein folding and quality control in the endoplasmic re

Brodsky, J. L., and W. R. Skach. (2011) Protein folding and quality control in the endoplasmic reticulum: recent lessons from yeast and mammalian systems.  Curr. Opin. Cell Biol. 23: 464-475

Huryn, D.M., J.L. Brodsky, K.M. Brummond, P. G. Chambers, B. Eyre, A.W. Ireland, M. Kawasumi, M.G

Huryn, D.M., J.L. Brodsky, K.M. Brummond, P. G. Chambers, B. Eyre, A.W. Ireland, M. Kawasumi, M.G. LaPorte, K. Lloyd, B. Manteau, P. Nghiem, B. Quade, S.P. Seguin, and P. Wipf.  (2011)  Chemical Methodology as a Source of Small Molecule Checkpoint Inhibitors and Hsp70 Modulators.  Proc. Natl. Acad. Sci. USA 108: 6757-6762

Bell, S. L., A. Chiang, and J. L. Brodsky. (2011). A malarial Hsp70 rescues chaperone-dependent a

Bell, S. L., A. Chiang, and J. L. Brodsky. (2011). A malarial Hsp70 rescues chaperone-dependent activities in ssa1 mutant yeast. PLoS One 6: e20047

Kolb, A. R., T. M. Buck, and J. L. Brodsky (2011) Saccharomyces cerevisiae as a model sy

Kolb, A. R., T. M. Buck, and J. L. Brodsky (2011) Saccharomyces cerevisiae as a model system for kidney disease: What can yeast tell us about renal function? Am. J. Physiol. 301: F1-F11

Yi, S.X., J.B. Benoit, M.A. Elnitsky, N. Kaufmann, J.L. Brodsky, M.L. Zeidel, D.L. Denlinger, and

Yi, S.X., J.B. Benoit, M.A. Elnitsky, N. Kaufmann, J.L. Brodsky, M.L. Zeidel, D.L. Denlinger, and J.r Lee RE (2011) Function and immuno-localization of aquaporins in the Antarctic midge Belgica antarctica. J Insect Physiol 57: 1096-1105

Tran, J.R., L.R. Tomsic, and J.L. Brodsky (2011) A Cdc48p-associated factor modulates endoplasmic

Tran, J.R., L.R. Tomsic, and J.L. Brodsky (2011) A Cdc48p-associated factor modulates endoplasmic reticulum-associated degradation, cell stress, and ubiquitinated protein homeostasis. J Biol Chem 286:5744-5755

Botha, M., A.N. Chiang, P.G. Needham, L.L. Stephens, H.C. Hoppe, S. Kulzer, J.M. Przyborski, K. L

Botha, M., A.N. Chiang, P.G. Needham, L.L. Stephens, H.C. Hoppe, S. Kulzer, J.M. Przyborski, K. Lingelbach, P. Wipf, J.L. Brodsky, A. Shonhai, and G.L. Blatch (2011) Plasmodium falciparum encodes a single cytosolic type I Hsp40 that functionally interacts with Hsp70 and is upregulated by heat shock. Cell Stress Chaperones 16: 389-401

Ahn, J.H., W. Luo, J. Kim, A. Rodina, C.C. Clement, J. Aguirre, W. Sun, Y. Kang, R. Maharaj, K. M

Ahn, J.H., W. Luo, J. Kim, A. Rodina, C.C. Clement, J. Aguirre, W. Sun, Y. Kang, R. Maharaj, K. Moulick, D. Zatorska, M. Kokoszka, J.L. Brodsky, and G. Chiosis (2011) Design of a Flexible Cell-Based Assay for the Evaluation of Heat Shock Protein 70 Expression Modulators. Assay Drug Dev Technol 9: 236-246

Gelling, C.L., and J.L. Brodsky (2010) Mechanisms underlying the cellular clearance of antitrypsi

Gelling, C.L., and J.L. Brodsky (2010) Mechanisms underlying the cellular clearance of antitrypsin Z: lessons from yeast expression systems. Proc Am Thorac Soc 7:363-367

Heo, J.M., N. Livnat-Levanon, E.B. Taylor, K.T. Jones, N. Dephoure, J. Ring, J. Xie, J.L. Brodsky

Heo, J.M., N. Livnat-Levanon, E.B. Taylor, K.T. Jones, N. Dephoure, J. Ring, J. Xie, J.L. Brodsky, F. Madeo, S.P. Gygi, K. Ashrafi, M.H. Glickman, and J. Rutter (2010) A stress-responsive system for mitochondrial protein degradation. Mol. Cell 40:465-480

Brodsky, J.L. (2010) The use of in vitro assays to measure endoplasmic reticulum-associated degra

Brodsky, J.L. (2010) The use of in vitro assays to measure endoplasmic reticulum-associated degradation. Methods Enzymol. 470:661-679

Brodsky, J.L. (2010) The special delivery of a tail-anchored protein: why it pays to use a dedica

Brodsky, J.L. (2010) The special delivery of a tail-anchored protein: why it pays to use a dedicated courier. Mol. Cell 40:5-7

Goeckeler, J.L., and J.L. Brodsky (2010) Molecular chaperones and substrate ubiquitination contro

Goeckeler, J.L., and J.L. Brodsky (2010) Molecular chaperones and substrate ubiquitination control the efficiency of endoplasmic reticulum-associated degradation. Diabetes Obes. Metab. 12 Sup:32-38

Wisen, S., E.B. Bertelsen, A.D. Thompson, S. Patury, P. Ung, L. Chang, C.G. Evans, G.M. Walter, P

Wisen, S., E.B. Bertelsen, A.D. Thompson, S. Patury, P. Ung, L. Chang, C.G. Evans, G.M. Walter, P. Wipf, H.A. Carlson, J.L. Brodsky, E.R. Zuiderweg, and J.E. Gestwicki (2010) Binding of a small molecule at a protein-protein interface regulates the chaperone activity of Hsp70-Hsp40. ACS Chem. Biol. 5:611-622

Vembar, S.S., M.C. Jonikas, L.M. Hendershot, J.S. Weissman, and J.L. Brodsky (2010) J domain co-c

Vembar, S.S., M.C. Jonikas, L.M. Hendershot, J.S. Weissman, and J.L. Brodsky (2010) J domain co-chaperone specificity defines the role of BiP during protein translocation. J. Biol. Chem. 285:22484-22494

Nakatsukasa, K., and J.L. Brodsky (2010) In vitro reconstitution of the selection, ubiqu

Nakatsukasa, K., and J.L. Brodsky (2010) In vitro reconstitution of the selection, ubiquitination, and membrane extraction of a polytopic ERAD substrate. Methods Mol. Biol. 619:365-376

Buck, T.M., A.R. Kolb, C.R. Boyd, T.R. Kleyman, and J.L. Brodsky (2010) The endoplasmic reticulum

Buck, T.M., A.R. Kolb, C.R. Boyd, T.R. Kleyman, and J.L. Brodsky (2010) The endoplasmic reticulum-associated degradation of the epithelial sodium channel requires a unique complement of molecular chaperones. Mol Biol Cell 21:1047-1058
Prof. Jeffrey Brodsky holds the Avinoff Chair in Biological Sciences at the University of Pittsburgh. He was born in Chicago, Illinois, and attended the University of Illinois where he received his bachelor's degree in Biochemistry, graduating magna cum laude with honors in 1985. He then entered the Biochemistry and Molecular Biology graduate program at Harvard University, receiving his PhD in 1990, and worked with Prof. Guido Guidotti on regulation of the sodium pump in the brain. Next, he moved to the University of California, Berkeley for post-doctoral research as an American Cancer Society Research Fellow and studied with Prof. Randy Schekman (Nobel Laureate, 2013), who pioneered the use of the yeast model to define how proteins are trafficked within the cell. Prof. Brodsky joined the Faculty in the Department of Biological Sciences at the University of Pittsburgh in 1994 and was promoted to Associate Professor in 2000. In 2006 he was promoted to Full Professor and was awarded the Avinoff Chair. Prof. Brodsky’s work now focuses on understanding how misfolded proteins are recognized and destroyed in the cell, and how defects in protein architecture can be corrected using drugs and genetic approaches. Based on his work, he received the University of Pittsburgh Chancellor's Outstanding Research Award in the Junior Division in 1998 and the Chancellor’s Outstanding Teaching Award in 2008, as well as the Pitt Innovator Award in 2007. In 2013, Dr. Brodsky was elected a Fellow of the American Association for the Advancement of Science (AAAS) and in 2022 he was again awarded the Chancellor's Outstanding Research Award (Senior Division). He also leads the Center for Protein Conformational Diseases on campus. Prof. Brodsky has served on the editorial boards of three journals, has published >250 scientific papers, holds 3 patents, and has acted as a scientific consultant for several disease foundations and biotech/pharmaceutical companies.