Graham Hatfull

  • Eberly Family Professor of Biotechnology, HHMI Professor
  • Phages and tuberculosis

Contact

Office: (412) 624-6975
Lab: (412) 624-6976
304 Clapp Hall
4249 Fifth Avenue
Pittsburgh, PA 15260

Mycobacteria are among some of the worst pathogens of Man. Mycobacterium tuberculosis – the causative agent of human TB – infects a substantial portion of the world’s population and is responsible for over 1.5 millions deaths a year. Nontuberculous mycobacteria (NTM) are important opportunistic pathogens and are relatively common in persons with cystic fibrosis and those that are immunosuppressed. Although TB can be treated with multidrug regimens, antibiotic resistance is becoming widespread, and new treatments are needed. NTM infections are intrinsically resistant to many antibiotics, leading to treatment failure and harmful consequences of long-term drug treatments. The study of mycobacteriophages – viruses that infect mycobacteria – can provide new tools for manipulating and understanding the mycobacteria, and the therapeutic use of phages shows considerable promise.  Bacteriophage discovery and genomics also offers insights into viral diversity, evolutions, and origins, and provides a powerful platform for science education.

Corndog: Mycobacteriophage Corndog

Here's just a flavor of some of the current studies going on in the lab:

Bacteriophage genomics. We have been examining bacteriophage genomes since 1988 and through the development of science education programs such as the Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program we have established a collection of over 20,000 individual phages isolated on bacteria with the phylum Actinobacteria, half of which were isolated on the strain Mycobacterium smegmatis mc2155. Over 4,000 of the genomes have been completely sequenced and annotated providing a high-resolution view of viral diversity and a wealth of insights into phage biology, diversity, and evolution. A database of these phages and their genomes is available at:  https://phagesdb.org.

Understanding mycobacteriophage biology. A curiosity of phage genomes is the high proportion of ‘novel ‘genes of unknown function. We are investigating gene functions using a variety of strategies including genetic studies using gene toxicities, gene expression approaches, and biochemical studies to understand protein activities. We are especially interested in the determinant of phage host range that determine which phages can efficiently infect different bacterial strains. A large and growing collection of clinical isolates of Mycobacterium abscessus is especially valuable for these studies.

Development of tools for genetics.  Phage genomes are rich toolboxes for development of new genetic tools.  We have developed a suite of integration-proficient plasmid vectors, new selectable markers, and recombineering strategies for use in both fast- and slow-growing mycobacteria.  We have also adapted these tools for genetic engineering of the phages, including Bacteriophage Recombineering of Electroporated DNA (BRED) and CRISPY-BRED, which adds a CRISPR-mediated counterselection to the BRED protocol.

Bacteriophage therapy. We are investigating the potential for phages to provide clinical benefits for patients with TB and NTM infections. We have reported the therapeutic use of phages in over 20 patients – mostly with M. abscessus infections – with favorable clinical or microbiological outcomes in a majority of patients. There are many challenges in broadening the potential for phage therapies and advancing from individual compassionate use interventions to clinical trials to understand safety and efficacy.

Complete publication list here

E-mail Lab

Schmalstig AA, Wiggins A, Badillo D, Wetzel KS,

Schmalstig AA, Wiggins A, Badillo D, Wetzel KS, Hatfull GF, Braunstein M. Bacteriophage infection and killing of intracellular Mycobacterium abscessus. mBio. 2023 Dec 7:e0292423. doi: 10.1128/mbio.02924-23. Epub ahead of print. PMID: 38059609.  

Podgorski JM, Podgorski J, Abad L, Jacobs-Sera

Podgorski JM, Podgorski J, Abad L, Jacobs-Sera D, Freeman KG, Brown C, Hatfull G, Luque A, White SJ. A novel stabilization mechanism accommodating genome length variation in evolutionarily related viral capsids. bioRxiv [Preprint]. 2023 Nov 3:2023.11.03.565530. doi: 10.1101/2023.11.03.565530. PMID: 37961133; PMCID: PMC10635136.

LaBianca K, Butura J, McKnight A, Pan K, Whelan

LaBianca K, Butura J, McKnight A, Pan K, Whelan S, Punska E, Pause R, Igbinosa B, Ribbe A, Chien P, Russell D, Garlena R, Hatfull G, Jacobs-Sera D, Rocheleau J. Complete genome sequence of phi29-like Microbacterium foliorum podovirus phage PineapplePizza. Microbiol Resour Announc. 2023 Oct 19;12(10):e0047823. doi: 10.1128/MRA.00478-23. Epub 2023 Sep 6. PMID: 37671874; PMCID: PMC10586137

Gauthier CH, Hatfull GF. (2023) PhamClust: a ph

Gauthier CH, Hatfull GF. (2023) PhamClust: a phage genome clustering tool using proteomic equivalence. mSystems. Oct 4:e0044323. doi: 10.1128/msystems.00443-23. Epub ahead of print. PMID: 37791778.

Abad L, Gauthier CH, Florian I, Jacobs-Sera D,

Abad L, Gauthier CH, Florian I, Jacobs-Sera D, Hatfull GF. (2023). The heterogenous and diverse population of prophages in Mycobacterium genomes. mSystems. Oct 4:e0044623. doi: 10.1128/msystems.00446-23. Epub ahead of print. PMID: 37791767.

Binsabaan, S. A., Freeman, K. G., Hatfull, G. F

Binsabaan, S. A., Freeman, K. G., Hatfull, G. F., and VanDemark, A. P. (2023). The cytotoxic mycobacteriophage protein Phaedrus gp82 interacts and modulates the activity of the host ATPase, MoxR. J. Mol Biol. Sep 9;435(20):168261. doi: 10.1016/j.jmb.2023.168261. Epub ahead of print. PMID: 37678706.

LaBianca, K., Butura, J., McKnight, A., Pan, K.

LaBianca, K., Butura, J., McKnight, A., Pan, K., Whelan, S., Punska, E., Pause. R. J., Igbinosa, B.,  Ribbe, A., Chien, P., Russell, D. A., Garlena, R. A., Hatfull, G. F., Jacobs-Sera, D., and Rocheleau, J., (2023). Complete Genome Sequence of phi29-like Microbacterium foliorum Podovirus phage PineapplePizza. Microbiol. Resour. Announc. In press.

Wetzel, K. S., Illouz, M., Abad, L., Aull, H. G

Wetzel, K. S., Illouz, M., Abad, L., Aull, H. G., Russell, D. A., Garlena, R. A., Cristinziano, M., Malmsheimer, S., Chalut, C., Hatfull, G. F., and Kremer, L. (2023). Therapeutically useful mycobacteriophages BPs and Muddy require trehalose polyphleates. Nature Microbiology. 8,1717-1731. doi: 10.1038/s41564-023-01451-6. PMID: 37644325; PMCID: PMC10465359.

Dedrick, R. M., Abad, L., Storey, N., Kaganovsk

Dedrick, R. M., Abad, L., Storey, N., Kaganovsky, A. M., Smith, B. E., Aull, H. E., Cristinziano, M.,  Morkowska, A., Murphy, S., Loebinger, M., Hatfull, G. F., and Satta, G.(2023). The problem of Mycobacterium abscessus complex: multidrug resistance, bacteriophage susceptibility and potential healthcare transmission. Clinical Microbiology and Infection Jun 24:S1198-743X(23)00304-X. doi: 10.1016/j.cmi.2023.06.026. PMID: 37364635.

Illouz, M., Leclercq, L-D., Dessenne, C., Hatfu

Illouz, M., Leclercq, L-D., Dessenne, C., Hatfull, G. F., Daher, W., Kremer, L. (2023). Multiple Mycobacterium abscessus O-acetyltransferases influence glycopeptidolipid structure.  J. Bio. Chem. J. Bio. Chem. Jun 28:104979. doi: 10.1016/j.jbc.2023.104979. PMID: 37390990

Freeman, K. G., Rob

Freeman, K. G., Robotham, A. C., Parks, O. B., Abad, L., Jacobs-Sera, D., Lauer, M. J., Podgorski, J. M.,  Zhang, Y., Williams, J. V., White, S. J. Kelly, J. K., Hatfull, G. F., and Pope, W. H., (2023) Virion glycosylation determines mycobacteriophage immune recognition. Cell Host and Microbe 31, 1216-1231.e6. doi: 10.1016/j.chom.2023.05.028. PMID: 37329881.

Hurst-Hess, K., Walz A., Yang, Y., McGuirk, H.,

Hurst-Hess, K., Walz A., Yang, Y., McGuirk, H., Gonzalez-Juarrero, M., Hatfull, G. F. Ghosh, P., and Ojha, A. K. (2023). Intrapulmonary treatment with mycobacteriophage LysB rapidly reduces Mycobacterium abscessus burden. Antimicrob Agents Chemother. 67:e0016223. doi: 10.1128/aac.00162-23. Epub 2023 May 8. PMID: 37154689; PMCID: PMC10269076.

Dulberger, C. L., Guerrero-Bustamante, C. A., O

Dulberger, C. L., Guerrero-Bustamante, C. A., Owen, S. V., Wilson, S., Wuo, M. G., Garlena, R. A., Serpa, L. A., Russell, D. A., Zhu, J., Braunecker, B. J., Squyres, G. R., Baym, M., Kiessling, L., Garner, E. C., Rubin, E. J., and Hatfull, G. F. (2023). Mycobacterial nucleoid-associated protein Lsr2 is required for productive mycobacteriophage infection. Nat Microbiol.  doi: 10.1038/s41564-023-01333-x. Epub ahead of print. PMID: 36823286

Hatfull, G. F. (2023). Phage Therapy for Nontub

Hatfull, G. F. (2023). Phage Therapy for Nontuberculous Mycobacteria: Challenges and Opportunities. Pulmonary Therapy https://doi.org/10.1007/s41030-022-00210-y

Amarh, E. D., Dedrick, R. M., Garlena, R. A., R

Amarh, E. D., Dedrick, R. M., Garlena, R. A., Russell, D. A., Gauthier, C. H., Aull, H. G., Abad, L., Jacobs-Sera, D., Akusobi, C., Rubin, E. J., and Hatfull, G. F. (2023) Unusual prophages in Mycobacterium abscessus genomes and strain variations in phage susceptibilities.  PLoS One. 16;18(2):e0281769. doi: 10.1371/journal.pone.0281769. PMID: 36795728; PMCID: PMC9934374

Strathdee, S. A., Hatfull, G. F., Mutalik, V. K

Strathdee, S. A., Hatfull, G. F., Mutalik, V. K., and Schooley, R. T. (2023). Phage Therapy: From Biologic Mechanisms to Future Directions.  Cell 186, 17-31. doi: 10.1016/j.cell.2022.11.017. PMID: 36608652; PMCID: PMC9827498.

Podgorski, J. M., Freeman, K., Gosselin, S., Hu

Podgorski, J. M., Freeman, K., Gosselin, S., Huet, A., Conway, J. F., Bird, M., Grecco, J., Patel, S., Jacobs-Sera, D., Hatfull, G., Gogarten, J. P., Ravantti, J., White, S. J. (2023). A structural dendrogram of the actinobacteriophage major capsid proteins provides important structural insights into the evolution of capsid stability. Structure. S0969-2126(22)00498-1. doi: 10.1016/j.str.2022.12.012. Epub ahead of print. PMID: 36649709.

Dedrick, R. M. Smith, B. E., Cristinziano, M.,

Dedrick, R. M. Smith, B. E., Cristinziano, M., Freeman, K. G., Jacobs-Sera, D., Belessis, Y., Brown, A. W., Cohen, K. A., Davidson, R. M., van Duin, D., Gainey, A., Berastegui Garcia, C., George, C. R. R., Haidar, G., Ip, W., Iredell, J., Khatami, A., Little, J. S., Malmivaara, K., McMullan, B. J., Michalik, D. E., Moscatelli, A., Nick , J. A., Tupayachi Ortiz, M. J., Polenakovik, H. M., Robinson, P. D., Skurnik, M., Solomon, D. A., Soothill, J., Spencer, H., Wark, P., Worth, A., Schooley, R. T., Benson, C. A., and Hatfull, G. F. (2023). Phage Therapy of Mycobacterium Infections: Compassionate-use of Phages in Twenty Patients with Drug-Resistant Mycobacterial Disease. Clinical Infectious Diseases. Jun 9:ciac453. doi: 10.1093/cid/ciac453

Feiss, M., Young, R., Ramsey, J., Adhya, S., Ge

Feiss, M., Young, R., Ramsey, J., Adhya, S., Georgopoulos, C., Hendrix, R. W., Hatfull, G. F., Gilcrease, E. B., & Casjens, S. R. (2022). Hybrid vigor: Importance of hybrid l phages in early insights in molecular biology. Microbiol. Mol. Biol. Rev. e0012421. doi: 10.1128/mmbr.00124-21. PMID: 36165780.

Gauthier, C. H., Cresawn, S. G., and Hatfull, G

Gauthier, C. H., Cresawn, S. G., and Hatfull, G. F. (2022). PhaMMseqs: A new pipeline for constructing phage gene phamilies using MMseqs2. G3: Genes, Genomes, Genetics. 12:jkac233. doi: 10.1093/g3journal/jkac233. PMID: 36161315

Hatfull, G. F.  (2022).  Mycobacterio

Hatfull, G. F.  (2022).  Mycobacteriophages: From Petri Dish to Patient.  PLoS Pathogens.  18:e1010602. doi: 10.1371/journal.ppat.1010602.

Gauthier, C. H., Abad, A., Venbakkam, A. K., Ma

Gauthier, C. H., Abad, A., Venbakkam, A. K., Malnak, J., Russell, D. A., and Hatfull, G.F. (2022). DEPhT: A novel approach for efficient prophage discovery and precise extraction. Nucleic Acids Res.  Nucleic Acids Res. 22:gkac273. doi: 10.1093/nar/gkac273.

Hanauer, D. I., Graham, M. J., Jacobs-Sera, D.,

Hanauer, D. I., Graham, M. J., Jacobs-Sera, D., Garlena, R. A., Russell, D. A., Sivanathan, V., Asai, D. J., and Hatfull, G. F. (2022). Broadening Access to STEM through the Community College: Investigating the Role of Course-Based Research Experiences (CRE). CBE Life Sci Educ. 21:ar38. doi: 10.1187/cbe.21-08-0203

Feiss, M., Adhya, S., Georgopoulos, C., Hendrix

Feiss, M., Adhya, S., Georgopoulos, C., Hendrix, R.W., Hatfull, G.F., Gilcrease, E.B., Casjens, S.R., Ramsey, J., Young, R. (2022). Complete Genome Sequences of Lambdoid Phages 21, 434, and 434B and Several Lambda Hybrids.  Microbiol Resour Announc. 2022 11:e0012022. doi: 10.1128/mra.00120-22.

Little, J. S., Dedrick, R. M., Freeman, K. G.,

Little, J. S., Dedrick, R. M., Freeman, K. G., Cristinziano, M. M., Smith, B. E., Benson, C. A., Jhaveri, T. A., Baden, L. R., Solomon, D. A., and Hatfull, G. F. (2022). Bacteriophage Treatment of Disseminated Cutaneous Mycobacterium chelonae Infection. Nat Comm 13, 2313. doi: 10.1038/s41467-022-29689-4.  

Nick, J. A., Dedrick, R. M., Gray, A. L., Vlada

Nick, J. A., Dedrick, R. M., Gray, A. L., Vladar, E. K., Smith, B. E., Freeman, K. G.,, Malcolm K. C., Epperson, L. E., Hasan, N. A., Hendrix., J., Callahan, K., Walton, K., Vestal, B., Wheeler, E., Rysavy, N., Poch, K., Caceres, S., Lovell, V. K., Hisert, K. B., Calado de Moura, V., Chatterjee, D., De, P., Weakly, N., Martiniano, S. L., Lynch, D. A., Daley, C. L., Strong, M. Fan Jia, F., Hatfull, G. F., Davidson, R. M. (2022) Host and Pathogen Response to Bacteriophage Engineered Against Mycobacterium abscessus Lung Infection. Cell 185, 1860-1874.e12. doi: 10.1016/j.cell.2022.04.024.  

Dedrick, R. M., Freeman, K. G., Nguyen, J. A.,

Dedrick, R. M., Freeman, K. G., Nguyen, J. A., Bahadirli-Talbott, A., Cardin, M. E., Cristinziano, M., Smith, B. E., Jeong, S., Ignatius, E. H., Lin, C. T., Cohen, K. A., & Hatfull, G. F. (2022). Nebulized bacteriophage in a patient with refractory Mycobacterium abscessus lung disease. Open Forum Infectious Diseases, ofac194, doi.org/10.1093/ofid/ofac194

Hanauer, D. I., Graham, M. J.,Arnold, R.J., Ayu

Hanauer, D. I., Graham, M. J.,Arnold, R.J., Ayuk, M.A., Balish, M.F., Beyer, A.R., Butela, K.A., Byrum, C.A., Chia, C.P., Chung, H.M., Clase, K.L., Conant, S., Coomans, R.J., D'Elia, T., Diaz, J., Diaz, A., Doty, J.A., Edgington, N.P., Edwards, D.C., Eivazova, E., Emmons, C.B., Fast, K.M., Fisher, E.J., Fleischacker, C.L., Frederick, G.D., Freise, A.C., Gainey, M.D., Gissendanner, C.R., Golebiewska, U.P., Guild, N.A., Hendrickson, H.L., Herren, C.D., Hopson-Fernandes, M.S., Hughes, L.E., Jacobs-Sera, D., Johnson, A.A., Kirkpatrick, B.L., Klyczek, K.K., Koga, A.P., Kotturi, H., LeBlanc-Straceski, J., Lee-Soety, J.Y., Leonard, J.E., Mastropaolo, M.D., Merkhofer, E.C., Michael, S.F., Mitchell, J.C., Mohan, S., Monti, D.L., Noutsos, C., Nsa, I.Y., Peters, N.T., Plymale, R., Pollenz, R.S., Porter, M.L., Rinehart, C.A., Rosas-Acosta, G., Ross, J.F., Rubin, M.R., Scherer, A.E., Schroeder, S.C., Shaffer, C.D., Sprenkle, A.B., Sunnen, C.N., Swerdlow, S.J., Tobiason, D., Tolsma, S.S., Tsourkas, P.K., Ward, R.E., Ware, V.C., Warner, M.H., Washington, J.M., Westover, K.M., White, S.J., Whitefleet-Smith, J.L., Williams, D.C., Wolyniak, M.J., Zeilstra-Ryalls, J.H., Asai, D.J., Hatfull, G.F., Sivanathan, V.  (2022). Instructional Models for Course-Based Research Experience (CRE) Teaching. CBE Life Sci Educ. 21:ar8. doi: 10.1187/cbe.21-03-0057.

Shield, C. G., Swift, B. M. C., McHugh, T. D.,

Shield, C. G., Swift, B. M. C., McHugh, T. D., Dedrick, R. M., Hatfull, G. F. and Satta, G.  (2021).  Application of Bacteriophages for Mycobacterial Infections, from Diagnosis to Treatment. Microorganisms 9, 2366. doi.org/10.3390/microorganisms9112366

Diacon, A. H., Guerrero-Bustamante, C. A., Rose

Diacon, A. H., Guerrero-Bustamante, C. A., Rosenkranz, B.,  Pomar, F. J. R., Vanker, N., and Hatfull, G. F.  (2021).  Mycobacteriophages to treat tuberculosis - dream or delusion? Respiration, 23, 1-15. doi: 10.1159/000519870.

Freeman, K. G., Wetzel, K. S., Zhang, Y., Zack,

Freeman, K. G., Wetzel, K. S., Zhang, Y., Zack, K. M., Jacobs-Sera, D., Walters,S. M., Barbeau, D. J., McElroy, A. K., Williams, J. V., and Hatfull, G. F. (2021). A mycobacteriophage-based vaccine platform: SARS-CoV-2 antigen expression and display. Microorganisms 9, 2414. doi.org/10.3390/microorganisms9122414. 

Johansen, M. D., Alcaraz, A., Dedrick, R. M., R

Johansen, M. D., Alcaraz, A., Dedrick, R. M., Roquet-Baneres, F., Hamela, C., Hatfull, G. F., & Kremer, L.  (2021). Mycobacteriophage-antibiotic synergism promotes enhanced bacterial clearance of multi-drug resistant Mycobacterium abscessus in a zebrafish model. Dis. Models Mech 14, doi: 10.1242/dmm.049159.

Hatfull, G.F. (2021) Wildy Prize Lecture, 2020-

Hatfull, G.F. (2021) Wildy Prize Lecture, 2020-2021: Who wouldn't want to discover a new virus?  Microbiology (Reading), 167. Doi: 10.1099/mic.0.001094. 

Dedrick, R. M., Freem

Dedrick, R. M., Freeman, K. G., Nguyen, J. A., Bahadirli-Talbott, A., Smith, B. E., Wu, A. E., Ong, A. S., Lin, C. T., Ruppel, L. C., Parrish, N. M., Hatfull. G. F. and Cohen, K. A.  (2021).  Potent antibody mediated neutralization limits bacteriophage treatment of a pulmonary Mycobacterium abscessus infection.  Nature Medicine.  Jul 8. doi: 10.1038/s41591-021-01403-9

Guerrero-Bustamante.

Guerrero-Bustamante. C.A., Dedrick, R. M., Garlena, R. A., Russell, D. A., & Hatfull, G. F.  (2021).  Towards a phage cocktail for tuberculosis: Susceptibility and tuberculocidal action of mycobacteriophages against diverse Mycobacterium tuberculosis strains.  mBio 12 e00973-21

Dedrick, R. M., Aull, H. G., Jacobs-Sera, D., Garl

Dedrick, R. M., Aull, H. G., Jacobs-Sera, D., Garlena, R. A., Russell, D. A., Smith, B. E., Mahalingam, V., Abad, L., Gauthier, C. H., & Hatfull, G. F. (2021). The prophage and plasmid mobilome as a likely driver of Mycobacterium abscessus diversity. mBio 12:e03441-20. doi: 10.1128/mBio.03441-20. PMID: 33785627

Dedrick, R. M., Smith, B. E., Garlena, R. A., Russ

Dedrick, R. M., Smith, B. E., Garlena, R. A., Russell, D. A., Aull, H. G., Mahalingam, V., Divens, A. M., Guerrero-Bustamante, C., Zack, K., Abad, L., Gauthier, C. H., Jacobs-Sera, D., & Hatfull, G. F. (2021). Mycobacterium abscessus strain morphotype determines phage susceptibility, the repertoire of therapeutically useful phages, and phage resistance. mBio 12:e03431-20. doi: 10.1128/mBio.03431-20. PMID: 33785625

Pezo, V., Jaziri, F., Bourguignon, P-Y., Louis,

Pezo, V., Jaziri, F., Bourguignon, P-Y., Louis, D., Jacobs-Sera, D., Rozenski, J. Pochet, S., Herdewijn, P., Hatfull, G. F., Kaminski, P. A., & Marliere, P. (2021). Non-canonical DNA polymerization by aminoadenine-based siphoviruses. Science 372, 520-524.

Amarh, E. D., Dedrick, R. M., Garlena, R. A., Russ

Amarh, E. D., Dedrick, R. M., Garlena, R. A., Russell, D. A., Jacobs-Sera, D., Hatfull, G. F. (2021). Genome Sequence of Mycobacterium abscessus Phage phiT45-1. Microbiol Resour Announc 10:e00155-21. doi: 10.1128/MRA.00155-21. PMID: 33707330.

Hatfull, G. F., Dedrick, R. M., & Schooley,

Hatfull, G. F., Dedrick, R. M., & Schooley, R.  (2021).  Phage Therapy for Antibiotic-Resistant Bacterial Infections.  Annual Rev. Med. doi: 10.1146/annurev-med-080219-122208.  

Judd, J., Canestrari, J., Clark, R., Joseph, A., L

Judd, J., Canestrari, J., Clark, R., Joseph, A., Lapierre, P., Lasek-Nesselquist, E., Mir, M., M. Palumbo, Smith, C., Stone,, M., Upadhyay, A., Wirth, S., Dedrick, R., Meier, C., Russell, D. A., Dills, A.,, Dove, E., Kester, J., Wolf, I., Zhu, J., Rubin, E., Fortune, S., Hatfull, G. F., Gray, T., Wade, J., & Derbyshire, K. M. (2021). A Mycobacterial Systems Resource for the Research Community. 12:e02401-20. doi: 10.1128/mBio.02401-20. PMID: 33653882

Wetzel, K. S., Guerrero-Bustamante, C. A., Dedrick

Wetzel, K. S., Guerrero-Bustamante, C. A., Dedrick, R. M., Ko C-C., Freeman, K. G., Aull, H. G., Divens, M. A. M., Rock, J. M., Zack, K. M., & Hatfull, G. F. (2021). CRISPY-BRED and CRISPY-BRIP: Efficient bacteriophage engineering. Sci. Reports. 24, 11:6796. doi: 10.1038/s41598-021-86112-6. PMID: 33762639

Zaworski, J., McClung, C., Ruse, C., Weigele, P. R

Zaworski, J., McClung, C., Ruse, C., Weigele, P. R., Hendrix, R. W., Ko, C-C., Edgar, R., Hatfull, G. F., Casjens, S. R., Raleigh, E. A. (2021). Genome analysis of Salmonella enterica serovar Typhimurium bacteriophage L, indicator for StySA (StyLT2III) restriction-modification system action. G3 Genes|Genomes|Genetics 11. https://doi.org/10.1093/g3journal/jkaa037. PMID: 33561243

Wetzel, K.S., Aull, H.G., Zack, K.M., Garlena, R.A

Wetzel, K.S., Aull, H.G., Zack, K.M., Garlena, R.A. & Hatfull, G.F. (2020) Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages. mBio 11. pii: e00385-20. doi: 10.1128/mBio.00385-20. PMID: 32209683

Jacobs-Sera, D., Abad, L. A., Alvey, R. M., Anders

Jacobs-Sera, D., Abad, L. A., Alvey, R. M., Anders, K.R., Aull, H. G., Bhalla, S. S., Blumer, L. A, Bollivar, D. W., Alfred Bonilla, J., Butela, K.A., Coomans, R. J., Cresawn, S. G. , D'Elia, T., Diaz, A., Divens, A. M., Edgington, N. P., Frederick, G. D., Gainey, M. D., Garlena, R. A., Grant, K.W., Gurney, S. M. R., Hendrickson, H. L., Hughes, L. E.18, Kenna, M. A., Klyczek, K. K., Kotturi, H., Mavrich, T. N., McKinney, A. L., Merkhofer, E. C., Parker, J. M., Molloy, S. D., Monti, D. L., Pape-Zambito, D. A., Pollenz, R. S., Pope, W. H., Reyna, N. S., Rinehart, C. A., Russell, D. A., Shaffer, C. D., Sivanathan, V., Stoner, T. H., Stukey, J., Sunnen, C. N., Tolsma, S. S., K. Tsourkas, P., Wallen, J. R., Ware, V. C., Warner. M. H., Washington, J. M., Westover, K. M., Whitefleet-Smith, J. L., Wiersma-Koch, H. I., Williams, D. C., Zack, K. M., and Hatfull, G. F. (2020) Genomic diversity of bacteriophages infecting Microbacterium spp. PLoS One 15(6), e0234636. PMID: 32555720

Ko, C. C. and Hatfull, G. F. (2020). Identificat

Ko, C. C. and Hatfull, G. F. (2020). Identification of mycobacteriophage toxic genes reveals new features of mycobacterial physiology and morphology. Sci Rep. Sep 4;10,14670. doi: 10.1038/s41598-020-71588-5. PMID: PMID: 32887931

Mavrich, T. N., Gauthier, C., Abad, L., Bowman, C.

Mavrich, T. N., Gauthier, C., Abad, L., Bowman, C. A., Cresawn, S. G., and Hatfull, G. F. (2020). pdm_utils: a SEA-PHAGES MySQL phage database management toolkit. Bioinformatics. Nov 23:btaa983. doi: 10.1093/bioinformatics/btaa983. PMID: 33226064

Hatfull, G. F.  (2020).  Actinobacter

Hatfull, G. F.  (2020).  Actinobacteriophages.  Annual Rev. Virol. 7, 37-61. doi: 10.1146/annurev-virology-122019-070009.

Podgorski, J., Calabrese, J., Alexandrescu, L., Ja

Podgorski, J., Calabrese, J., Alexandrescu, L., Jacobs-Sera, D., Pope, W., Hatfull, G. F., & White, S. (2020). Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins. Viruses 12. pii: E294. doi: 10.3390/v12030294. PMID: 32182721

Amarh, E. D., Dedrick, R. M., Garlena, R. A., Russ

Amarh, E. D., Dedrick, R. M., Garlena, R. A., Russell, D. A., Jacobs-Sera, D., Hatfull, G. F. (2021). Genome Sequence of Mycobacterium abscessus Phage phiT46-1. Microbiol Resour Announc 10. Doi: 10.1128/MRA.01421-20. PMID: 33446600

Pope, W. H., Butela, K. A., Garlena, R. A., Jacobs

Pope, W. H., Butela, K. A., Garlena, R. A., Jacobs-Sera, D., Russell, D. A., Warner, M.H. University of Pittsburgh SEA-PHAGES, and Hatfull, G. F. (2020). Genome Sequences of 20 Bacteriophages Isolated on Gordonia terrae. Microbial. Resource Announcements, 16 pii: e01489-19. doi: 10.1128/MRA.01489-19. PMID: 31948974

Carrigy, N. B., Larsen, S. E., Reese, V., Pecor, T

Carrigy, N. B., Larsen, S. E., Reese, V., Pecor, T., Harrison, M., Kuehl, P. J., Hatfull, G. F. Sauvageau, D., Finlay, W. H., Coler, R. N., and Vehring, R. (2019). Prophylaxis of Mycobacterium tuberculosis H37Rv Infection in a Preclinical Mouse 1 Model via Inhalation of Nebulized Bacteriophage D29. Antimicrobial. Agents Chemo. pii: AAC.00871-19. doi: 10.1128/AAC.00871-19. PMID: 31527037

Hutinet, G., Kot, W., Cui, L., Hillebrand, R., Bal

Hutinet, G., Kot, W., Cui, L., Hillebrand, R., Balamkundu, S., Gnanakalai, S., Neelakandan., R., Carstens, A. B., Chuan., L., Tremblay, D., Jacobs-Sera, D., Sassanfar. M., Weigele, P., Moineau, S. Hatfull, G. F., Dedon, P. C., Hansen, L. H., and de Crécy-Lagard. V. (2019). 7-Deazaguanine modifications protect phage DNA from host restriction systems. Nat. Commun. 10, 5442. doi: 10.1038/s41467-019-13384-y. PMID: 31784519

Evolution of Superinfection Immunity in Cluster

Evolution of Superinfection Immunity in Cluster A Mycobacteriophages.  (2019).  Mavrich, T.N. and Hatfull, G.F.  mBio. 2019 Jun 4; 10 (3). pii: e00971-19. doi: 10.1128/mBio.00971-19.  PMID:  31164468

Gauthier CH, Hatfull GF. (2023). PhamClust: a p

Gauthier CH, Hatfull GF. (2023). PhamClust: a phage genome clustering tool using proteomic equivalence.  mSystems. Oct 4:e0044323. doi: 10.1128/msystems.00443-23. Epub ahead of print. PMID: 37791778.

Oliveira, H., Sampaio, M., Melo, L.

Oliveira, H., Sampaio, M., Melo, L. D. R., Dias, O., Pope, W. H., Hatfull, G. F. and Azeredo, J.  (2019).  Staphylococci phages display vast genomic diversity and evolutionary relationships.  BMC Genomics 20, 357. doi: 10.1186/s12864-019-5647-8.  PMID: 31072320

Dedrick, R. M., Guerrero-Bustamante, C. A., Gar

Dedrick, R. M., Guerrero-Bustamante, C. A., Garlena, R. A., Russell, D. A., Ford, K., Harris, K., Gilmour, K. C., Soothill, J., Jacobs-Sera, D., Schooley, R. R., Hatfull, G. F. and Spencer, H.  (2019).  Use of engineered bacteriophages for personalized treatment of a patient with a disseminated drug resistant Mycobacterium abscessus infection.  Nature Med. 25, 730-733. doi: 10.1038/s41591-019-0437-z.  PMID: 31068712

Mycobacteriophage Z

Mycobacteriophage ZoeJ: A broad host-range close relative of mycobacteriophage TM4.  (2019).  Dedrick, R.M., Guerrero Bustamante, C.A., Garlena, R.A., Pinches, R.S., Cornely, K. and Hatfull, G.F.  Tuberculosis (Edinb). 115:14-23. doi: 10.1016/j.tube. 2019.01.002.  PMID: 30948168
Dr. Hatfull received his Ph.D. in 1981 with Willie Donachie at the University of Edinburgh, Scotland, performed postdoctoral studies with Nigel Grindley at Yale University and with Fred Sanger at the MRC, and joined the Department in 1988. https://en.wikipedia.org/wiki/Graham_Hatfull