Title: Understanding the role of INO80 complex in murine embryonic stem cells
Abstract:
Transcription, as with all DNA-templated activities, is highly regulated, partially through the tight packaging of eukaryotic DNA into the nucleus in the form of chromatin. Nucleosomes, the basic unit of chromatin, are composed of a histone octamer wrapped by approximately 150 bp of DNA. For transcription to occur, the transcriptional machinery must have access to DNA. Regulation comes in many forms, but one class of important regulatory enzymes are nucleosome remodelers. Nucleosome remodelers translocate DNA within nucleosomes to provide a more accessible or inaccessible DNA template for transcription. Nucleosome remodelers are highly conserved proteins in eukaryotes and defined by their SNF2-helicase domains. One such remodeler is Inositol auxotropy 80 complex (INO80C).
To better understand the role of INO80C in mammalian cells we have generated and are generating precise murine embryonic stem (ES) cell lines within the Ino80, Arp5, and Ies6 subunits of the INO80C. These cell lines are informed by both yeast and structure studies, and will be used to address precisely how the INO80 complex is acting in a mammalian system to drive appropriate transcription. Specifically, I will assess how these mutations contribute to ES cell phenotypes, including growth assays, stem cell pluripotency assessment, and RNA-seq. Based on these results, I will then design downstream analyses for the most interesting candidates, including chromatin characterization. Together this work will lead to a better understanding of INO80C in mammalian cells.
Hainer Lab
Friday, March 1st, 2024
12:00PM
Langley A219B