Graham Hatfull

  • Eberly Family Professor of Biotechnology, HHMI Professor
  • Phages and tuberculosis

Contact

Office: (412) 624-6975
Lab: (412) 624-6975
376 Crawford Hall
4249 Fifth Avenue
Pittsburgh, PA 15260

Mycobacterium tuberculosis kills more people than any other single infectious agent. Since antibiotics are available and the BCG vaccine is in widespread use, why do two million people die each year from TB? The answer, in part, is that we really don't understand this curious bacterium or what parts of its genetic instructions make this such a deadly pathogen. At the heart of our strategies to understand mycobacterial genetics is the mycobacteriophages - viruses that infect the mycobacteria. These are easy to grow and manipulate and offer advantages over working with the slow-growing mycobacteria (such as M. tuberculosis) that can take up to a month to produce a colony on an agar plate. Phages are also rich sources of  potential genetic and molecular tools that can be used to study - and to modify - their bacterial hosts.

Corndog: Mycobacteriophage Corndog

Here's just a flavor of some of the current studies going on in the lab:

    Exploring bacteriophage genomics. In collaboration with Dr. Hendrix we have spearheaded an initiative to understand viral diversity and evolution. Our specific focus is on the genomic characterization of mycobactriophages, and a collection of about 250 complete genome sequences have been determined. Many of these phages were isolated and sequenced through three programs in which phage discovery and genomics is a platform for integrating our science and educational missions. These are the Pittsburgh Phage Hunters Integrating Research and Education (PHIRE) program, the Howard Hughes Medical Institute Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (HHMI SEA-PHAGES) program, and a Univiersity of KwaZulu-Natal and KwaZulu-Nalat Research in TB and HIV (UKZN/K-RITH) workshop.  These studies have not only provided valuable insights into phage diveristy and evolution, but present a rich and easily-accessible reservoir of genetic and mechanistic novelty for further study. A database of mycobaectriophage genomic infomration is available at http://www.phagesdb.org.

      Exploiting mycobacteriophages. We are dissecting the mycobacteriophages to understand the functional roles of the thousands of genes we have identified, and to deternine if and when they are expressed, and how this expression is regulated.  We are exploiting this information to develop tools and approaches that not only generate new tools for genetic manipulation for tuberculosis, but also to gain advances in diagnosis, prevention and treatment of the disease.

      Site-specific recombination. Many if not most of the mycobacteriophages we have sequenced integrate their DNA into the host chromosome (and can excise them too). We are studying the mechanism of integrase-mediated site-specific recombination with a primary current focus on the serine-integrases.  We are partiualrly interested in understanding how recomibnational directionality is determine in phage integration systems.

      Tools - Genetic and Clinical. Studying the mycobacteria and their phages has great potential for the development of novel tools for their genetics but also for a more direct clinical involvement. Two systems we have been involved in developing are multivalent recombinant BCG vaccines and Luciferase Reporter Phages, but there are numerous additional strategies awaiting further development!

      E-mail Lab

      Pope, W. H. & Hatfull, G. F. (2016). Adding

      Pope, W. H. & Hatfull, G. F. (2016). Adding pieces to the puzzle: New insights into bacteriophage diversity from integrated research-education programs.  Bacteriophage 5(4):e1084073.

      Hatfull, G. F. (2016). In the Trenches of Micro

      Hatfull, G. F. (2016). In the Trenches of Microbial Warfare: Identification of Genes and Pathways Contributing to Bacteriophage Infection by Quantitative Selection Analysis. J. Mol. Biol. 428, 413-415.

      Hanauer, D. I & Hatfull, G. F. (2015).&nbsp

      Hanauer, D. I & Hatfull, G. F. (2015).  Measuring Networkign as an Outcome Variable in Undergraduate Research Experiecnes.  CBE Life Sci Educ. 14(4):ar38

      Villanueva, V. M., Oldfield, L. M. & 

      Villanueva, V. M., Oldfield, L. M. &  Hatfull, G. F. (2015). An unusual phage repressor ecoded by mycobacteriophage BPs.  PLoSOne 10(9):e0137187

      Petrova, Z. O., Broussard, G. W. and Hatfull, G

      Petrova, Z. O., Broussard, G. W. and Hatfull, G. F. (2015). Mycobacteriophage-repressor mediated immunity as selectable genetic markers: Adephagia and BPs repressor-selection.  Microbiology In press.

      Mehla, J., R.M. Ded

      Mehla, J., R.M. Dedrick, J.H. Caufield, R. Siefring, M. Mair, A. Johnson, G.F. Hatfull & P. Uetz, (2015) The protein interactome of mycobacteriophage Giles predicts functions for unknown proteins. J Bacteriol. 197, 2508-2516.

      Pope, W.H., T. Ahme

      Pope, W.H., T. Ahmed, M.K. Drobitch, D.R. Early, S. Eljamri, N.S. Kasturiarachi, E.F. Klonicki, D.T. Manjooran, A.N. Ni Chochlain, A.O. Puglionesi, V. Rajakumar, K.A. Shindle, M.T. Tran, B.R. Brown, B.M. Churilla, K.L. Cohen, K.E. Wilkes, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequences of Mycobacteriophages Luchador and Nerujay. Genome announcements 3.

      Pope, W.H., D.A. Au

      Pope, W.H., D.A. Augustine, D.C. Carroll, J.C. Duncan, K.M. Harwi, R. Howry, B. Jagessar, B.A. Lum, J.W. Meinert, J.S. Migliozzi, K.A. Milliken, C.J. Mitchell, A.S. Nalatwad, K.C. Orlandini, M.J. Rhein, V. Saravanan, B.A. Seese, J.G. Schiebel, K.B. Thomas, N.L. Adkins, K.L. Cohen, V.B. Iyengar, H. Kim, Z.J. Kramer, M.T. Montgomery, C.E. Schafer, K.E. Wilkes, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequences of Cluster G Mycobacteriophages Cambiare, FlagStaff, and MOOREtheMARYer. Genome announcements 3.

      Pope, W.H., N.I. Be

      Pope, W.H., N.I. Bernstein, C.S. Fasolas, N. Mezghani, C.A. Pressimone, P. Selvakumar, A.C. Stanton, J.S. Lapin, A.K. Prout, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of Mycobacteriophage Mindy. Genome announcements 3.

      Pope, W.H., E.A. Bi

      Pope, W.H., E.A. Bina, I.S. Brahme, A.B. Hill, P.H. Himmelstein, S.M. Hunsicker, A.R. Ish, T.S. Le, M.M. Martin, C.N. Moscinski, S.A. Shetty, T. Swierzewski, V.B. Iyengar, H. Kim, C.E. Schafer, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of Mycobacteriophage Momo. Genome announcements 3.

      Pope, W.H., M.E. Ca

      Pope, W.H., M.E. Carbonara, H.M. Cioffi, T. Cruz, B.Q. Dang, A.N. Doyle, O.H. Fan, M. Gallagher, G.M. Gentile, B.A. German, M.E. Farrell, M. Gerwig, K.L. Hunter, V.E. Lefever, N.A. Marfisi, J.E. McDonnell, J.K. Monga, K.G. Quiroz, A.C. Pong, P.A. Rimple, M. Situ, P.C. Sohnen, A.N. Stockinger, P.K. Thompson, N.M. Torchio, C.L. Toner, M.C. Ulbrich, N.I. Vohra, A. Zakir, N.L. Adkins, B.R. Brown, B.M. Churilla, Z.J. Kramer, J.S. Lapin, M.T. Montgomery, A.K. Prout, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequences of Mycobacteriophages AlanGrant, Baee, Corofin, OrangeOswald, and Vincenzo, New Members of Cluster B. Genome announcements 3.

      Pope, W.H., J.T. Ca

      Pope, W.H., J.T. Carter, K.L. Dasher, M.C. Haynberg, A. Reddi, K.A. Shedlock, J.S. Lapin, A.K. Prout, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of a Newly Isolated Mycobacteriophage, ShedlockHolmes. Genome announcements 3.

      Pope, W.H., E. Jaco

      Pope, W.H., E. Jacobetz, C.A. Johnson, B.L. Kihle, M.A. Sobeski, M.B. Werner, N.L. Adkins, Z.J. Kramer, M.T. Montgomery, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of Mycobacteriophage Phayonce. Genome announcements 3.

      Hatfull, G.F. (2015

      Hatfull, G.F. (2015). Dark matter of the biosphere: The amazing world of bacteriophage diversity. J. Virol. 89, 8107-8110.

      Hatfull, G.F. (2015

      Hatfull, G.F. (2015). Innovations in Undergraduate Science Education: Going Viral. J Virol. 89, 8111-8113.

      Mehla, J., Dedrick, R.M., Caufield, J.H., Siefr

      Mehla, J., Dedrick, R.M., Caufield, J.H., Siefring, R., Mair, M., Johnson, A., Hatfull, G.F., and Uetz, P. (2015). The protein interactome of mycobacteriophage Giles predicts functions for unknown proteins. J. Bacteriol. pii: JB.00164-15

      Pope, W.H., Bowman, C.A., Russell, D.A., Jacobs

      Pope, W.H., Bowman, C.A., Russell, D.A., Jacobs-Sera, D., Asai, D.J., Cresawn, S.G., Jacobs, W.R., Hendrix, R.W., Lawrence, J.G., Hatfull, G.F., et al. (2015). Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity. Elife 4.

      Ojha, A. K., Jacobs, W R. Jr., and Hatfull, G.

      Ojha, A. K., Jacobs, W R. Jr., and Hatfull, G. F.  (2015). Genetic Dissection of Mycobacterial Biofilms in Mycobacteria Protocols. Methods in Molecular Biology 1285, 215-226.

      Casjens S. R.

      Casjens S. R., Jacobs-Sera, D., Hatfull, G. F., Hendrix, R. W. (2015) Genome sequence of Salmonella enterica phage Det7.  Genome Announc.  May 7;3(3). pii: e00279-15. doi: 10.1128/genomeA.00279-15.

      Cresawn, S. G., Pope, W. H., Jacobs-Sera, D., B

      Cresawn, S. G., Pope, W. H., Jacobs-Sera, D., Bowman, C. A., Russell, D. A., Dedrick, R. A., Adair, T., Anders, K. R., Ball, S., Bollivar, D., Breitenberger, C., Burnett, S. H., Butela, K., Byrnes, D., Carzo, S., Cornely, K. A., Cross, T., Daniels, R. L., Dunbar, D., Findley, A. M., Gissendanner, C. R., Golebiewska, U. P., Hartzog, G. A., Hatherill, J. R., Hughes, L. E., Jalloh, C. S., DeLosSantos, C., Ekanam, K., Khambule, S. L., King, R. A., King-Smith, C., Klyczek, K., Krukonis, G. P., Laing, C., Lapin, J. S., Lopez, A. J., Mkhwanazi, S. M., Molloy, S. D., Moran, D., Munsamy, V., Pacey, E., Plymale, R., Poxleitner, M., Reyna, N., Schildbach, J. F., Stukey, J., Taylor, S. E., Ware, V. C., Wellmann, A. L., Westholm, D., Wodarski, D., Zajko, M., Zikalala, T. S., Hendrix, R. W., and  Hatfull, G. F. (2015) Comparative Genomics of Cluster O Mycobacteriophages PLoS One 10:e0118725.

      Hendrix, R. W., Ko, C. C., Jacobs-Sera, D., Hat

      Hendrix, R. W., Ko, C. C., Jacobs-Sera, D., Hatfull, G. F., Erhardt, M., Hughes, K. T,, Casjensm S. R. (2015) Genome Sequence of Salmonella Phage χ. Genome Announc. 3. pii: e01229-14.

      Pope, W. H., Jacobs-Sera, D., Russell, D. A., R

      Pope, W. H., Jacobs-Sera, D., Russell, D. A., Rubin, D. H. F., Kajee, A., Msibi, Z. N. P., Larsen, M. H., Jacobs, W. R. Jr., Lawrence, J. G., Hendrix, R. W., & Hatfull, G. F. (2014). Genomics and proteomics of Mycobacteriophage Patience: An accidental tourist in the Mycobacterium neighborhood mBio pii: e02145-14. doi: 10.1128/mBio.02145-14.

      Hatfull, G. F. (2014) Molecular

      Hatfull, G. F. (2014) Molecular Genetics of Mycobacteriophages. Microbiol Spectr.  2, 1-36.

      Hatfull, G. F. and Racaniello, V. (2014). PHIRE

      Hatfull, G. F. and Racaniello, V. (2014). PHIRE and TWiV: Experiences in bringing virology to new audiences. Ann. Rev. Virology.  1, 37-53.

      Dieterle, M. E., Bowman, C., Batthyany, C., Lan

      Dieterle, M. E., Bowman, C., Batthyany, C., Lanzarotti, E., Turjanski, A., Hatfull, G., Piuri, M. 2014. Exposing the secrets of two well known Lactobacillus casei phages: Genomic and structural analysis of J-1 and PL-1. Appl. Environ. Microbiol.  80, 7107-7021

      Casjens, S. R., Leavitt, J. C., Ha

      Casjens, S. R., Leavitt, J. C., Hatfull, G. F., and Hendrix, R. W. 2014. Genome Sequence of Salmonella Phage 9NA. GenomeA pii: e00531-14

      Hatfull G. F. & Jacobs W.R Jr.(eds)  M

      Hatfull G. F. & Jacobs W.R Jr.(eds)  Molecular Genetics of Mycobacteria.  (2014). Second edition. ASM Press.  Washington, DC.

      Oldfield, L. M. and Hatfull, G. F. (2014). Muta

      Oldfield, L. M. and Hatfull, G. F. (2014). Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J. Bacteriol. 196, 3589-3597.

      Hatfull, G. F. (2014). Molecular Genetics of My

      Hatfull, G. F. (2014). Molecular Genetics of Mycobacteriophages in Molecular Genetics of Mycobacteria, Second Edition. Eds. Hatfull, G. F. & Jacobs, W. R. Jr. ASM press, Washington, DC.

      Tufariello, J. M., Malek, A. A., Vilchèze, C.,

      Tufariello, J. M., Malek, A. A., Vilchèze, C., Cole, L. E., Ratner, H. K., González, P. A., Jain, P., Hatfull, G. F., Larsen, M. H., and Jacobs, W. R. Jr. (2014) Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis. mBio 5(3):e01179-14

      Russell, D. A., Bowman, C. A., and Hatfull, G.

      Russell, D. A., Bowman, C. A., and Hatfull, G. F. (2014) Genome Sequence of Salmonella enterica subsp. enterica Strain Durban. Genome Announcements. 2(3). pii: e00399-14

      Hatfull, G. F. (2014). Mycobacteriophages: Wind

      Hatfull, G. F. (2014). Mycobacteriophages: Windows into tuberculosis.  PLoS Pathogens. 10(3):e1003953

      Jordan,T. C., Burnett, S. H., Carson, S., Carus

      Jordan,T. C., Burnett, S. H., Carson, S., Caruso, S. M., Clase, K., DeJong, R. J., Dennehy, J. J., Denver, D. R., Dunbar, D., Elgin, S. C. R., Findley A. M., Gissendanner, C. R., Golebiewska, U. P., Guild, N., Hartzog, G. A., Grillo, W. H., Hollowell, G. P., Hughes, L. E., Johnson, A., King, R. A., Lewis, L. O., Li,W., Rosenzweig, F., Rubin, M. R., Saha, M. S., Sandoz, J., Shaffer, C. D., Taylor, B., Temple,  L., Vazquez, E., Ware, V. C., Barker, L.P., Bradley, K. W., Jacobs-Sera, D., Pope, W. H., Russell, D. A., Cresawn, S. G., Lopatto, D., Bailey, C. P., Hatfull, G. F. (2014) A broadly implementable research course for first-year undergraduate students. mBio 5:e01051-01013.

      Pope, W.H., Anders, K. R., Baird, M., Bowman, C

      Pope, W.H., Anders, K. R., Baird, M., Bowman, C.A., Boyle, M.M., Broussard, G.W., Chow, T., Clase, K.L., Cooper, S., Cornely, K.A., Dejong, R.J., Delesalle, V.A., Deng, L., Dunbar, D., Edgington, N.P., Ferreira, C.M., Hafer, K.W., Hartzog, G.A., Hatherill, J.R., Hughes, L.E., Ipapo, K., Krukonis, G.P., Meier, C.G., Monti, D.L., Olm, M.R., Page, S.T., Peebles, C.L., Rinehart, C.A., Rubin, M.R., Russell, D.A., Sanders, E.R.,Schoer, M., Shaffer, J., Wherley, C.D.,Vazquez,E., Yuan, H., Zhang, D., Cresawn, S.G., Jacobs-Sera, D., Hendrix, R.W. & Hatfull, G. F. (2014) Cluster M mycobacteriophages Bongo, PegLeg, and Rey with unusually large repertoires of tRNA isotypes. J. Virol. 88, 2461-2480.

      Dieterle, M. E., Jacobs-Sera, D., Russell, D.,

      Dieterle, M. E., Jacobs-Sera, D., Russell, D., Hatfull, G., Piuri, M. (2014) Complete Genome Sequences of Lactobacillus Phages J-1 and PL-1. Genome Announc 2.

      Singh, S., Plaks, S. G., Homa, N. J., Amrich, C

      Singh, S., Plaks, S. G., Homa, N. J., Amrich, C. G., Heroux, A., Hatfull, G. F., & VanDemark, A. P. (2014). The structure of Xis reveals the basis for filament fomration and insight into DNA bending within a mycobacteriophage Intasome.  J. Mol. Biol., 426, 412-422.

      Singh. S., Rockenbach, K., Dedrick, R. M., VanD

      Singh. S., Rockenbach, K., Dedrick, R. M., VanDemark, A. P., & Hatfull, G. F. (2014) Cross-talk between Diverse Serine Integrases. J Mol Biol 426, 318-331.

      Smith, M. C., Hendrix, R. W., Dedrick, R., Mitc

      Smith, M. C., Hendrix, R. W., Dedrick, R., Mitchell, K., Ko, CC., Russell, D., Bell, E., Gregory, M., Bibb, M. J., Pethick, F., Jacobs-Sera, D., Herron, P., Buttner, M. J., and Hatull, G. F. (2013).  Evolutionary relationships within actinophages and a putative adaptation for growth in Streptomyces spp. J. Bacteriol. 195, 4924-4935.

      Hatfull, G. F. Science Education Alliance Phage

      Hatfull, G. F. Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program, KwaZulu-Natal Research Institute for Tuberculosis and HIV (K-RITH) Mycobacterial Genetics Course, University of California Los Angeles  Research Immersion Laboratory in Virology, Phage Hunters Integrating Research and Education (PHIRE) program. (2013). The complete genome sequences of 63 mycobacteriophages.  GenomeA 1, doi:pii: e00847-13. 10.1128/genomeA.00847-13.

      Piuri, M., Rondon, L., Urdaniz, E., and Hatfull

      Piuri, M., Rondon, L., Urdaniz, E., and Hatfull, G. F. (2013). Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids. Appl Environ Microbiol. 79, 5608-5615.

      Pope, W. H., Jacobs-Sera, D., Best, A. A., Brou

      Pope, W. H., Jacobs-Sera, D., Best, A. A., Broussard, G. W., Connerly, P. L., Dedrick, R. M., Kremer, T. A., Offner, S., Ogiefo, A. H., Pizzorno, M. C., Rockenbach, K., Russell, D. A., Stowe, E. L., Stukey, J., Thibault, S. A., Conway, J. F., Hendrix, R. W., Hatfull, G. F. (2013) Cluster J mycobacteriophages: intron splicing in capsid and tail genes. PLoS One 8:e69273.

      Broussard, G. W. and Hatfull, G. F. (2013) Evol

      Broussard, G. W. and Hatfull, G. F. (2013) Evolution of genetic switch complexity. Bacteriophage 3:e24186.

      Singh, S., Ghosh, P., and Hatfull, G. F. (2013)

      Singh, S., Ghosh, P., and Hatfull, G. F. (2013).  Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination.  PLoS Genetics.  9, e1003490

      Sambandan, D., D.N. Dao, B.C. Weinrick, C. Vilc

      Sambandan, D., D.N. Dao, B.C. Weinrick, C. Vilcheze, S.S. Gurcha, A. Ojha, L. Kremer, G.S. Besra, G.F. Hatfull & W.R. Jacobs, Jr., (2013) Keto-mycolic acid-dependent pellicle formation confers tolerance to drug-sensitive Mycobacterium tuberculosis. MBio 4: e00222-00213.

      Hatfull, G. F. (Ed). Recent Progress in Bacteri

      Hatfull, G. F. (Ed). Recent Progress in Bacteriophage Research (2013).  Multidisciplinary Digital Publishing Institute, Basel.

      Silva, J.L., M. Piuri, G. Broussard, L. Marinel

      Silva, J.L., M. Piuri, G. Broussard, L. Marinelli, G.M. Bastos, R.D. Hirata, G.F. Hatfull & M.H. Hirata, (2013) Application of BRED technology to construct recombinant D29 reporter phage expressing EGFP. FEMS Microbiol Lett. 344, 166-172.

      Dedrick, R. M., Marinelli, L. J., Newton, G. L.

      Dedrick, R. M., Marinelli, L. J., Newton, G. L., Pogliano, K., Pogliano, J ., Hatfull, G. F. (2013). FUnctional requirements for bacetriophage growth: gene essentiality and expression in mycobacteriophage Giles. Mol. Microbiology  EPub head of print.   

      Sencilo, A., Jacobs-Sera, D., Russell, D. A., K

      Sencilo, A., Jacobs-Sera, D., Russell, D. A., Ko, C-C., Bowman, C. A., Atanasova, N., Österlund, E., Oksanen, H. M., Bamford, D. H., Hatfull, G. F., Roine, E., & Hendrix, R.W.  (2013).  Snapshot of haloarchaeal tailed virus genomes.  RNA Biology.  In Press

      Broussard, G.W., L.M. Oldfield, V.M. Villanueva

      Broussard, G.W., L.M. Oldfield, V.M. Villanueva, B.L. Lunt, E.E. Shine & G.F. Hatfull, (2013) Integration-Dependent Bacteriophage Immunity Provides Insights into the Evolution of Genetic Switches. Mol Cell. 49, 237-248.

      Jacobs-Sera, D., Marinelli, L. J., Bowman, C.,

      Jacobs-Sera, D., Marinelli, L. J., Bowman, C., Broussard, G. W., Guerrero Bustamante, C., Boyle, M. M., Petrova, Z. O., Dedrick, R. M., Pope, W. H., Science Education Alliance Phage Hunters Advancing, G., Evolutionary Science Sea-Phages, P., Modlin, R. L., Hendrix, R. W., and Hatfull, G. F. (2012) On the nature of mycobacteriophage diversity and host preference. Virology 434, 187-201.

      Marinelli, L.J., Fitz-Gibbon, S., Hayes, C., Bo

      Marinelli, L.J., Fitz-Gibbon, S., Hayes, C., Bowman, C., Inkeles, M., Loncaric, A., Russell, D.A., Jacobs-Sera, D., Cokus, S., Pellegrini, M., Kim, J., Miller, J. F., Hatfull, G. F., and Modlin, R. L. (2012). Propionibacterium acnes Bacteriophages Display Limited Genetic Diversity and Broad Killing Activity against Bacterial Skin Isolates. mBio 3. e00279-12

      Bai, H., Kath, J.E., Zorgiebel, F.M., Sun, M.,

      Bai, H., Kath, J.E., Zorgiebel, F.M., Sun, M., Ghosh, P., Hatfull, G.F., Grindley, N.D., and Marko, J.F. (2012). Remote control of DNA-acting enzymes by varying the Brownian dynamics of a distant DNA end. Proc Natl Acad Sci U S A. Epub

      Marinelli, L. J. , Hatfull, G. F., Piuri, M. (2

      Marinelli, L. J. , Hatfull, G. F., Piuri, M. (2012) Recombineering: A powerful tool for modification of bacteriophage genomes.  Bacteriophage 2, 5-14. 

      Payne, K.M., and Hatfull, G.F. (2012). Mycobact

      Payne, K.M., and Hatfull, G.F. (2012). Mycobacteriophage endolysins: diverse and modular enzymes with multiple catalytic activities. PLoS One 7, e34052

      Mageeney, C., W. Pope, M. Harrison, D. Moran, T

      Mageeney, C., W. Pope, M. Harrison, D. Moran, T. Cross, D. Jacobs-Sera, R. W. Hendrix, D. Dunbar & G. F. Hatfull, (2012) Mycobacteriophage Marvin: A new singleton phage with an unusual genome organization. J Virol. 86:4762-75.

      Hatfull (2012).  The Secret Lives of Mycob

      Hatfull (2012).  The Secret Lives of Mycobacteriophages. Advances in Virus Research. 82,179-288.

      Hatfull, G. F., et al (2012) Complete genome sequences of 138 mycobacteriophages.  J. Virol. 86: 2382-2384.

      Hatfull, G. F., et al (2012) Complete genome sequences of 138 mycobacteriophages.  J. Virol. 86: 2382-2384.

      Kulka, K., Hatfull, G., and Ojha, A.K. (2012).

      Kulka, K., Hatfull, G., and Ojha, A.K. (2012). Growth of Mycobacterium tuberculosis biofilms. J. Vis. Exp. 15:3820

      Savinov, A., Pan, J., Ghosh, P. and Hatfull, G. F. (2012) The Bxb1 gp47 recombination directionality factor is required not only for prophage excision but also for phage DNA replication.  Gene 495: 42-48.

      Savinov, A., Pan, J., Ghosh, P. and Hatfull, G. F. (2012) The Bxb1 gp47 recombination directionality factor is required not only for prophage excision but also for phage DNA replication.  Gene 495: 42-48.

      Jain, P., Hartman, T.E., Eisenberg, N., O'Donne

      Jain, P., Hartman, T.E., Eisenberg, N., O'Donnell, M. R., Kriakov, J., Govender, K., Makume, M., Thaler, D.S., Maiga, M., Timmins, G.S., Bishai, W.R., Hatfull, G.F., Sturm, A.W., Larsen, M.H., Moodley, P., and Jacobs, W.R. (2012) A high intensity fluorophage enables detection and rapid drug susceptibility testing of Mycobacterium tuberculosis directy from sputum samples.  J. Clin. Microbiol. 50, 1362-1369

      Cresawn, S.G., Bogel, M., Day, N., Jacobs-Sera, D., Hendrix, R.W. and Hatfull, G.F. Phamerator: a

      Cresawn, S.G., Bogel, M., Day, N., Jacobs-Sera, D., Hendrix, R.W. and Hatfull, G.F. (2011) Phamerator: a bioinformatic tool for comparative bacteriophage genomics. BMC Bioinformatics 12:395.

      Anderson, W.A., Amasino, R.M., Ares, M., Jr., Banerjee, U., Bartel, B., Corces, V.G., Drennan, C.

      Anderson, W.A., Amasino, R.M., Ares, M., Jr., Banerjee, U., Bartel, B., Corces, V.G., Drennan, C.L., Elgin, S.C., Epstein, I.R., Fanning, E., Guillette, L.J., Jr., Handelsman, J., Hatfull, G.F., Hoy, R.R., Kelley, D., Leinwand, L.A., Losick, R., Lu, Y., Lynn, D.G., Neuhauser, C., ODowd, D.K., Olivera, T., Pevzner, P., Richards-Kortum, R.R., Rine, J., Sah, R.L., Strobel, S.A., Walker, G.C., Walt, D.R., Warner, I.M., Wessler, S., Willard, H.F. and Zare, R.N. (2011) Competencies: a cure for pre-med curriculum. Science 334: 760-761

      Pope, W.H., Ferreira, C.M., Jacobs-Sera, D., Benjamin, R.C., Davis, A.J., DeJong, R.J., Elgin, S.

      Pope, W.H., Ferreira, C.M., Jacobs-Sera, D., Benjamin, R.C., Davis, A.J., DeJong, R.J., Elgin, S.C.R., Guilfoile, F.R., Forsyth, M.H., Harris, A.D., Harvey, S.E., Hughes, L.E., Hynes, P.M., Jackson, A.S., Jalal, M.D., MacMurray, E.A., Manley, C.M., McDonough, M.J., Mosier, J.L., Osterbann, L.J., Rabinowitz, H.S., Rhyan, C.N., Russell, D.A., Saha, M.S., Shaffer, C.D., Simon, S.E., Sims, E.F., Tovar, I.G., Weisser, E.G., Wertz, J.T., Weston-Hafer, K.A., Williamson, K.E., Zhang, B., Cresawn, S.G., Jain, P., Piuri, M., Jacobs, W.R., Jr., Hendrix, R.W. and Hatfull, G.F. (2011) Cluster K Mycobacteriophages: Insights into the Evolutionary Origins of Mycobacteriophage TM4. PLoS ONE 6:e26750.

      Hatfull, G.F. and Hendrix, R.W. (2011) Bacteriophages and their Genomes. Current Opinions in

      Hatfull, G.F. and Hendrix, R.W. (2011) Bacteriophages and their Genomes. Current Opinions in Virology 1: 298-303

      Bai, H., Sun, M., Ghosh, P., Hatfull, G.F., Grindley, N.D. and Marko, J.F. (2011) Single-molecule

      Bai, H., Sun, M., Ghosh, P., Hatfull, G.F., Grindley, N.D. and Marko, J.F. (2011) Single-molecule analysis reveals the molecular bearing mechanism of DNA strand exchange by a serine recombinase. Proc Natl Acad Sci U S A 108:7419-24.

      Huang, J., Ghosh, P., Hatfull, G.F. and Hong, Y. (2011) Successive and targeted DNA integrations

      Huang, J., Ghosh, P., Hatfull, G.F. and Hong, Y. (2011) Successive and targeted DNA integrations in the Drosophila genome by Bxb1 and phiC31 integrases. Genetics 189:391-5.

      Marine, R., Polson, S.W., Ravel, J., Hatfull, G., Russell, D., Sullivan, M., Syed, F., Dumas, M.

      Marine, R., Polson, S.W., Ravel, J., Hatfull, G., Russell, D., Sullivan, M., Syed, F., Dumas, M. and Wommack, K.E. (2011) Evaluation of a transposase protocol for rapid generation of shotgun high-throughput sequencing libraries from nanogram quantities of DNA. Appl Environ Microbiol 77:  8071-9.

      Jain, P., Thaler, D.S., Maiga, M., Timmins, G.S., Bishai, W.R., Hatfull, G.F., Larsen, M.H. and J

      Jain, P., Thaler, D.S., Maiga, M., Timmins, G.S., Bishai, W.R., Hatfull, G.F., Larsen, M.H. and Jacobs, W.R. (2011) Reporter phage and breath tests: emerging phenotypic assays for diagnosing active tuberculosis, antibiotic resistance, and treatment efficacy. J Infect Dis 204: 1142-1150.

      Anderson, W.A., Banerjee, U., Drennan, C.L., Elgin, S.C., Epstein, I.R., Handelsman, J., Hatfull,

      Anderson, W.A., Banerjee, U., Drennan, C.L., Elgin, S.C., Epstein, I.R., Handelsman, J., Hatfull, G.F., Losick, R., ODowd, D.K., Olivera, B.M., Strobel, S.A., Walker, G.C. and Warner, I.M. (2011) Science education. Changing the culture of science education at research universities. Science 331:152-153.

      Pope, W.H., D. Jacobs-Sera, D.A. Russell, C.L. Peebles, Z. Al-Atrache, T.A. Alcoser, L.M. Alexand

      Pope, W.H., D. Jacobs-Sera, D.A. Russell, C.L. Peebles, Z. Al-Atrache, T.A. Alcoser, L.M. Alexander, M.B. Alfano, S.T. Alford, N.E. Amy, M.D. Anderson, A.G. Anderson, A.A. Ang, J.r Ares M, A.J. Barber, L.P. Barker, J.M. Barrett, W.D. Barshop, C.M. Bauerle, I.M. Bayles, K.L. Belfield, A.A. Best, J.r Borjon A, C.A. Bowman, C.A. Boyer, K.W. Bradley, V.A. Bradley, L.N. Broadway, K. Budwal, K.N. Busby, I.W. Campbell, A.M. Campbell, A. Carey, S.M. Caruso, R.D. Chew, C.L. Cockburn, L.B. Cohen, J.M. Corajod, S.G. Cresawn, K.R. Davis, L. Deng, D.R. Denver, B.R. Dixon, S. Ekram, S.C. Elgin, A.E. Engelsen, B.E. English, M.L. Erb, C. Estrada, L.Z. Filliger, A.M. Findley, L. Forbes, M.H. Forsyth, T.M. Fox, M.J. Fritz, R. Garcia, Z.D. George, A.E. Georges, C.R. Gissendanner, S. Goff, R. Goldstein, K.C. Gordon, R.D. Green, S.L. Guerra, K.R. Guiney-Olsen, B.G. Guiza, L. Haghighat, G.V. Hagopian, C.J. Harmon, J.S. Harmson, G.A. Hartzog, S.E. Harvey, S. He, K.J. He, K.E. Healy, E.R. Higinbotham, E.N. Hildebrandt, J.H. Ho, G.M. Hogan, V.G. Hohenstein, N.A. Holz, V.J. Huang, E.L. Hufford, P.M. Hynes, A.S. Jackson, E.C. Jansen, J. Jarvik, P.G. Jasinto, T.C. Jordan, T. Kasza, M.A. Katelyn, J.S. Kelsey, L.A. Kerrigan, D. Khaw, J. Kim, J.Z. Knutter, C.C. Ko, G.V. Larkin, J.R. Laroche, A. Latif, K.D. Leuba, S.I. Leuba, L.O. Lewis, K.E. Loesser-Casey, C.A. Long, A.J. Lopez, N. Lowery, T.Q. Lu, V. Mac, I.R. Masters, J.J. McCloud, M.J. McDonough, A.J. Medenbach, A. Menon, R. Miller, B.K. Morgan, P.C. Ng, E. Nguyen, K.T. Nguyen, E.T. Nguyen, K.M. Nicholson, L.A. Parnell, C.E. Peirce, A.M. Perz, L.J. Peterson, R.E. Pferdehirt, S.V. Philip, K. Pogliano, J. Pogliano, T. Polley, E.J. Puopolo, H.S. Rabinowitz, M.J. Resiss, C.N. Rhyan, Y.M. Robinson, L.L. Rodriguez, A.C. Rose, J.D. Rubin, J.A. Ruby, M.S. Saha, J.W. Sandoz, J. Savitskaya, D.J. Schipper, C.E. Schnitzler, A.R. Schott, J.B. Segal, C.D. Shaffer, K.E. Sheldon, E.M. Shepard, J.W. Shepardson, M.K. Shroff, J.M. Simmons, E.F. Simms, B.M. Simpson, K.M. Sinclair, R.L. Sjoholm, I.J. Slette, B.C. Spaulding, C.L. Straub, J. Stukey, T. Sughrue, T.Y. Tang, L.M. Tatyana, S.B. Taylor, B.J. Taylor, L.M. Temple, J.V. Thompson, M.P. Tokarz, S.E. Trapani, A.P. Troum, J. Tsay, A.T. Tubbs, J.M. Walton, D.H. Wang, H. Wang, J.R. Warner, E.G. Weisser, S.C. Wendler, K.A. Weston-Hafer, H.M. Whelan, K.E. Williamson, A.N. Willis, H.S. Wirtshafter, T.W. Wong, P. Wu, Y. Yang, B.C. Yee, D.A. Zaidins, B. Zhang, M.Y. Zuniga, R.W. Hendrix, and G.F. Hatfull (2011) Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution. PLoS One 6:e16329e16329

      Rondon, L., M. Piuri, J.r Jacobs WR, J. de Waard, G.F. Hatfull, and H. Takiff (2011) Evaluation o

      Rondon, L., M. Piuri, J.r Jacobs WR, J. de Waard, G.F. Hatfull, and H. Takiff (2011) Evaluation of Fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. J Clin Microbiol :

      Traag, B.A., A. Driks, P. Stragier, W. Bitter, G. Broussard, G. Hatfull, F. Chu, K.N. Adams, L. R

      Traag, B.A., A. Driks, P. Stragier, W. Bitter, G. Broussard, G. Hatfull, F. Chu, K.N. Adams, L. Ramakrishnan, and R. Losick (2010) Do mycobacteria produce endospores? Proc Natl Acad Sci U S A 107:878-881

      Hatfull, G.F. (2010) Mycobacteriophages: genes and genomes. Annu Rev Microbiol 6

      Hatfull, G.F. (2010) Mycobacteriophages: genes and genomes. Annu Rev Microbiol 64:331-356

      Alibaud, L., A. Alahari, X. Trivelli, A.K. Ojha, G.F. Hatfull, Y. Guerardel, and L. Kremer (2010)

      Alibaud, L., A. Alahari, X. Trivelli, A.K. Ojha, G.F. Hatfull, Y. Guerardel, and L. Kremer (2010) Temperature-dependent regulation of mycolic acid cyclopropanation in saprophytic mycobacteria: role of the Mycobacterium smegmatis 1351 gene (MSMEG_1351) in CIS-cyclopropanation of alpha-mycolates. J Biol Chem 285:21698-21707

      Ojha, A.K., X. Trivelli, Y. Guerardel, L. Kremer, and G.F. Hatfull (2010) Enzymatic hydrolysis of

      Ojha, A.K., X. Trivelli, Y. Guerardel, L. Kremer, and G.F. Hatfull (2010) Enzymatic hydrolysis of trehalose dimycolate releases free mycolic acids during mycobacterial growth in biofilms. J Biol Chem 285:17380-17389

      Sampson, T., G.W. Broussard, L.J. Marinelli, D. Jacobs-Sera, M. Ray, C.C. Ko, D. Russell, R.W. He

      Sampson, T., G.W. Broussard, L.J. Marinelli, D. Jacobs-Sera, M. Ray, C.C. Ko, D. Russell, R.W. Hendrix, and G.F. Hatfull (2009) Mycobacteriophages BPs, Angel and Halo: comparative genomics reveals a novel class of ultra-small mobile genetic elements. Microbiology 155:2962-2977

      Payne, K., Q. Sun, J. Sacchettini, and G.F. Hatfull (2009) Mycobacteriophage Lysin B is a novel m

      Payne, K., Q. Sun, J. Sacchettini, and G.F. Hatfull (2009) Mycobacteriophage Lysin B is a novel mycolylarabinogalactan esterase. Mol. Microbiol. 73:367-381

      Stewart, C.R., S.R. Casjens, S.G. Cresawn, J.M. Houtz, A.L. Smith, M.E. Ford, C.L. Peebles, G.F.

      Stewart, C.R., S.R. Casjens, S.G. Cresawn, J.M. Houtz, A.L. Smith, M.E. Ford, C.L. Peebles, G.F. Hatfull, R.W. Hendrix, W.M. Huang, and M.L. Pedulla (2009) The genome of Bacillus subtilis bacteriophage SPO1. J. Mol. Biol. 388:48-70

      Comeau, A.M., G.F. Hatfull, H.M. Krisch, D. Lindell, N.H. Mann, and D. Prangishvili (2008) Explor

      Comeau, A.M., G.F. Hatfull, H.M. Krisch, D. Lindell, N.H. Mann, and D. Prangishvili (2008) Exploring the prokaryotic virosphere. Res. Microbiol. 159:306-313

      Hatfull, G.F., S.G. Cresawn, and R.W. Hendrix (2008) Comparative genomics of the mycobacteriophag

      Hatfull, G.F., S.G. Cresawn, and R.W. Hendrix (2008) Comparative genomics of the mycobacteriophages: insights into bacteriophage evolution. Res. Microbiol. 159:332-339

      van Kessel, J.C., L.J. Marinelli, and G.F. Hatfull (2008) Recombineering mycobacteria and their p

      van Kessel, J.C., L.J. Marinelli, and G.F. Hatfull (2008) Recombineering mycobacteria and their phages. Nat. Rev. Microbiol. 6:851-857

      Marinelli, L.J., M. Piuri, Z. Swigonova, A. Balachandran, L.M. Oldfield, K.e.s.s.e. van, and G.F.

      Marinelli, L.J., M. Piuri, Z. Swigonova, A. Balachandran, L.M. Oldfield, K.e.s.s.e. van, and G.F. Hatfull (2008) BRED: a simple and powerful tool for constructing mutant and recombinant bacteriophage genomes. PLoS ONE 3:e3957

      van Kessel, J.C., and G.F. Hatfull (2008) Efficient point mutagenesis in mycobacteria using singl

      van Kessel, J.C., and G.F. Hatfull (2008) Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets. Mol. Microbiol. 67:1094-1107
      Dr. Hatfull received his Ph.D. in 1981 with Willie Donachie at the University of Edinburgh, Scotland, performed postdoctoral studies with Nigel Grindley at Yale University and with Fred Sanger at the MRC, and joined the Department in 1988.