Alex Francette- Arndt Lab
Active Contributions of Paf1C to Nascent Transcription
Most coding and non-coding regions of eukaryotic genomes are actively transcribed by RNA Polymerase II (RNAPII). One critical elongation factor, Polymerase Associated Factor 1 complex (Paf1C), associates with RNAPII starting early into transcription elongation and is known to play multifunctional roles in chromatin and transcriptional regulation. Using budding yeast as a model system, I have employed rapid protein depletion technologies and nascent transcriptomic methods with the goal of discerning the direct role of the constituent subunits of Paf1C in the control of transcription genome-wide. Analysis of two out five Paf1C subunits through nascent RNA-sequencing has revealed pervasive and genome-wide effects in gene expression when Paf1C function is depleted or impaired. Interestingly, tagging of the Paf1 subunit of Paf1C appears to contribute a hypomorphic phenotype that decreases transcriptomic output, but can still function to promote the deposition of the histone modification H2Bub. Future work will discriminate the active roles of Paf1C subunits in regulating transcription and chromatin maintenance.
Friday, September 25, 2020
12 PM
A219B or via ZoomÂ